Expression of a nonribosomal peptide synthetase-nonreducing polyketide synthase hybrid gene pcr10109 from Penicillium crustosum PRB-2 in Aspergillus nidulans led to the accumulation of 4-hydroxy-6-(4-hydroxyphenyl)-α-pyrone (1). Adding para-hydroxybenzoic acid into the medium in which the overexpressing mutant is growing increased the product yield up to 5-fold. This strategy could be helpful for heterologous gene expression experiments requiring special substrates for product formation.

中文翻译：

---

NRPS-PKS 基因表达后前体供应增加了 4-Hydroxy-6-(4-hydroxyphenyl)-α-pyrone 的积累

来自贝壳青霉PRB-2的非核糖体肽合成酶非还原性聚酮化合物合酶杂合基因pcr10109在构巢曲霉中的表达导致 4-羟基-6-(4-羟基苯基)-α-吡喃酮的积累 ( 1 )。向过度表达突变体生长的培养基中添加对羟基苯甲酸可使产物产量增加至多 5 倍。这种策略可能有助于需要特殊底物来形成产物的异源基因表达实验。