ABSTRACT

Clear cell renal cell carcinoma (ccRCC) is a subtype of renal cell cancer with the highest mortality, infiltration, and metastasis rate, threatening human health. Despite oncogenic role of TROAP in various cancers, its function in ccRCC remains to be unraveled. The differentially expressed mRNAs (DEmRNAs) and miRNAs (DEmiRNAs) were obtained by analyzing the related data sets of ccRCC in TCGA. The expression levels of mRNAs and miRNAs in the cell were detected by qRT-PCR, while the protein levels were characterized by western blot. The viability, migratory and invasive abilities of ccRCC cells were determined by MTT, wound healing and cell invasion assays. The combination of miRNA target site prediction and dual-luciferase reporter gene assay verified the binding relationship between miR-532-3p and TROAP. Research on ccRCC displayed that TROAP expression was upregulated, while miR-532-3p was down-regulated. Besides, upregulation of TROAP could accelerate viability, migratory and invasive potentials of ccRCC cells. On the contrary, miR-532-3p could downregulate TROAP level, but TROAP upregulation reversed the viability, migration, and invasion of ccRCC cells. MiR-532-3p could attenuate the viability, migration and invasion of ccRCC cells by targeting TROAP. This may generate novel insights into molecular therapeutic targets for ccRCC.

摘要

透明细胞肾细胞癌（ccRCC）是肾细胞癌的一种亚型，死亡率、浸润率和转移率最高，威胁人类健康。尽管 TROAP 在各种癌症中具有致癌作用，但其在 ccRCC 中的作用仍有待阐明。通过分析TCGA中ccRCC的相关数据集，获得差异表达的mRNAs（DEmRNAs）和miRNAs（DEmiRNAs）。通过qRT-PCR检测细胞中mRNA和miRNA的表达水平，而蛋白质水平通过蛋白质印迹表征。ccRCC细胞的活力、迁移和侵袭能力通过MTT、伤口愈合和细胞侵袭测定来确定。miRNA靶位点预测和双荧光素酶报告基因检测相结合验证了miR-532-3p与TROAP的结合关系。对ccRCC的研究表明，TROAP表达上调，而miR-532-3p下调。此外，TROAP的上调可以加速ccRCC细胞的活力、迁移和侵袭潜力。相反，miR-532-3p 可以下调 TROAP 水平，但 TROAP 上调会逆转 ccRCC 细胞的活力、迁移和侵袭。MiR-532-3p 可通过靶向 TROAP 减弱 ccRCC 细胞的活力、迁移和侵袭。这可能会对 ccRCC 的分子治疗靶点产生新的见解。MiR-532-3p 可通过靶向 TROAP 减弱 ccRCC 细胞的活力、迁移和侵袭。这可能会对 ccRCC 的分子治疗靶点产生新的见解。MiR-532-3p 可通过靶向 TROAP 减弱 ccRCC 细胞的活力、迁移和侵袭。这可能会对 ccRCC 的分子治疗靶点产生新的见解。