In this paper, hydrochlorothiazide (HCT) and losartan potassium (LSP) in tablets are simultaneously determined with UV-Vis spectrophotometry and chemometrics without separation. The spectra of standard and sample solutions were recorded in the wavelength from 220 to 300 nm at 1.0 nm intervals. The concentrations of HCT and LSP in the sample solutions were computed with the Kalman filter algorithm written on the Microsoft Excel 2016 and Visual Basic for Applications (VBA) platform. The method validation was determined via the accuracy and repeatability of measurements when analyzing HCT and LSP in the Splozarsin Plus tablet and comparing the mean values of their contents in the sample with those analyzed with HPLC. The proposed method is simple with a low cost compared with the standard HPLC method.

中文翻译：

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紫外-可见分光光度法和卡尔曼滤波法同时测定药品中氢氯噻嗪和氯沙坦钾

本文采用紫外-可见分光光度法和化学计量学不分离地同时测定片剂中的氢氯噻嗪 (HCT) 和氯沙坦钾 (LSP)。标准溶液和样品溶液的光谱在 220 到 300 nm 的波长范围内以 1.0 nm 的间隔记录。样品溶液中 HCT 和 LSP 的浓度使用在 Microsoft Excel 2016 和 Visual Basic for Applications (VBA) 平台上编写的卡尔曼滤波器算法计算。在分析 Splozarsin Plus 片剂中的 HCT 和 LSP 并将其含量的平均值与使用 HPLC 分析的平均值进行比较时，通过测量的准确性和可重复性来确定方法验证。与标准 HPLC 方法相比，所提出的方法简单且成本低。