Traumatic brain injury (TBI) remains a major cause of disability and death in modern society. In this study, we explored the neuroprotection role of the combination of gangliosides (GM) and mild hypothermia (MH) and the potential effect on oxidative stress injuries in a rat model of TBI. All 50 rats were randomized to five groups: (1) NC group: undergoing surgery without hit; (2) TBI group: undergoing surgery with hit; (3) GM group: TBI treated with gangliosides; (4) MHT group: TBI treated with MH; (5) GM+MHT group: TBI treated with gangliosides and MH. Spatial learning impairments, neurological function injury, Evans Blue leakage, brain MRI and oxidative stress injuries were assessed. The protein levels of Cleaved-caspase 3 and CytC were also detected. Both GM and MHT could rescue TBI-induced spatial learning impairments, improve neurological function injury and brain edema. In addition, the combination of them has a better therapeutic effect. Through the MRI, we found that compared with the TBI group, the brain tissue edema area of GM group, MHT group, and GM+MHT group was smaller, the occupancy effect was weakened, and the midline was slightly shifted. Compared with the GM group and MHT group, these changes in the GM+MHT group were much smaller. GM combined with MH-alleviated TBI-induced oxidative stress injuries and apoptosis. Our study reveals that GM and MH potentially provide neuroprotection via the suppression of oxidative stress injuries and apoptosis after TBI in rats.

中文翻译：

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神经节苷脂与亚低温相结合可在大鼠脑外伤模型中提供神经保护。

创伤性脑损伤（TBI）仍然是现代社会残疾和死亡的主要原因。在这项研究中，我们探讨了神经节苷脂 (GM) 和亚低温 (MH) 组合的神经保护作用以及对 TBI 大鼠模型氧化应激损伤的潜在影响。将50只大鼠随机分为5组：（1）NC组：接受手术，未受到打击；(2)TBI组：受伤后进行手术；(3)GM组：神经节苷脂治疗TBI；(4)MHT组：用MH治疗TBI；(5)GM+MHT组：用神经节苷脂和MH治疗TBI。评估了空间学习障碍、神经功能损伤、伊文思蓝渗漏、脑部 MRI 和氧化应激损伤。还检测了 Cleaved-caspase 3 和 CytC 的蛋白水平。GM和MHT均可以挽救TBI引起的空间学习障碍，改善神经功能损伤和脑水肿。另外，两者联合使用具有更好的治疗效果。通过MRI发现，与TBI组相比，GM组、MHT组、GM+MHT组脑组织水肿面积较小，占据效应减弱，中线略有偏移。与GM组和MHT组相比，GM+MHT组的这些变化要小得多。GM 联合 MH 减轻 TBI 诱导的氧化应激损伤和细胞凋亡。我们的研究表明，GM 和 MH 可能通过抑制大鼠 TBI 后的氧化应激损伤和细胞凋亡来提供神经保护。