In situ missions of exploration require analytical methods that are capable of detecting a wide range of molecular targets in complex matrices without a priori assumptions of sample composition. Furthermore, these methods should minimize the number of reagents needed and any sample preparation steps. We have developed a method for the detection of metabolically relevant inorganic and organic anions that is suitable for implementation on in situ spaceflight missions. Using 55 mM acetic acid, 50 mM triethylamine, and 5% glycerol, more than 21 relevant anions are separated in less than 20 min. The method is robust to sample ionic strength, tolerating high concentrations of background salts (up to 900 mM NaCl and 300 mM MgSO₄). This is an important feature for future missions to ocean worlds. The method was validated using a culture of Escherichia coli and with high salinity natural samples collected from Mono Lake, California.

中文翻译：

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用于分析与可居住性和寻找生命有关的无机和有机阴离子的毛细管电泳方法

原位探索任务需要能够检测复杂基质中的各种分子靶标的分析方法，而无需先验假设样品组成。此外，这些方法应尽量减少所需试剂的数量和任何样品制备步骤。我们开发了一种检测与代谢相关的无机和有机阴离子的方法，该方法适用于原位太空飞行任务。使用 55 mM 乙酸、50 mM 三乙胺和 5% 甘油，可在 20 分钟内分离超过 21 个相关阴离子。该方法对样品离子强度稳定，可耐受高浓度背景盐（高达 900 mM NaCl 和 300 mM MgSO ₄）。这是未来海洋世界任务的一个重要特征。该方法使用大肠杆菌培养物和从加利福尼亚州莫诺湖收集的高盐度天然样品进行了验证。