This study presents selective and efficient extraction of a medicinally important compound rosmarinic acid (RA) via molecular imprinting. Molecularly imprinted polymer (MIP) and its respective non-imprinted polymer (NIP) were synthesized using methacrylamide (MAAm), itaconic acid (IA), and 4-vinyl-1,3-dioxolan-2-one (VD) as functional monomers, and trimethylolpropane trimethacrylate (TRIM) as a crosslinker via bulk polymerization, and characterized by SEM, BET, FT-IR, and TGA. The adsorption properties of MIP were evaluated by batch rebinding experiments, Scatchard analysis, LF-isotherm, and kinetic models. The MIP showed higher adsorption capacity towards RA compared to the corresponding NIP: 41.9 mg/g compared to only 9.6 mg/g. In addition, the synthesized RA-MIP displayed a good selectivity towards RA compared to its structural analogs in competitive recognition studies, confirming the development of RA specific imprinting sites. HPLC was utilized to determine the concentration of RA and analogous compounds in competitive recognition tests and in the real sample analysis. Finally, RA-MIP was successfully utilized for the selective enrichment of RA from the ethanolic extract of Salvia hypoleuca. The obtained results show that RA-MIP can be a promising alternative to conventional extraction methods for the selective extraction and enrichment of RA from complex plant matrices.

本研究提出了通过分子印迹选择性和有效提取具有药用价值的化合物迷迭香酸 (RA)。使用甲基丙烯酰胺 (MAAm)、衣康酸 (IA) 和 4-vinyl-1,3-dioxolan-2-one (VD) 作为功能单体合成了分子印迹聚合物 (MIP) 及其各自的非印迹聚合物 (NIP)和三羟甲基丙烷三甲基丙烯酸酯 (TRIM) 作为通过本体聚合的交联剂，并通过 SEM、BET、FT-IR 和 TGA 进行表征。MIP 的吸附特性通过批量再结合实验、Scatchard 分析、LF 等温线和动力学模型进行评估。与相应的 NIP 相比，MIP 对 RA 的吸附容量更高：41.9 mg/g，而仅为 9.6 mg/g。此外，在竞争性识别研究中，合成的 RA-MIP 与其结构类似物相比对 RA 显示出良好的选择性，证实了 RA 特定印记位点的发展。在竞争性识别测试和实际样品分析中，HPLC 用于确定 RA 和类似化合物的浓度。最后，RA-MIP 成功地用于从乙醇提取物中选择性富集 RA。鼠尾草。获得的结果表明 RA-MIP 可以作为传统提取方法的有前途的替代方法，用于从复杂植物基质中选择性提取和富集 RA。