The efficient separation and purification methods are the major obstacles in the production and further use of bioproducts. One such valuable bioproduct is the thermostable C-phycocyanin (C-PC). There is a great commercial need to develop C-PC production with an appropriate degree of purity. However, the selected methods and their sequences are also depended on the quality of crude extract obtained from biomass. In this paper three down-stream processing methods, such as foam fractionation (FF), which according to our knowledge has not been used for separation of C-PC, aqueous two-phase extraction (ATPE) and conventional ultrafiltration were investigated for purification of thermostable C-PC from two crude extracts with lower and higher protein concentration and purity obtained from biomass of Synechococcus sp. PCC6715. The FF process resulted in a recovery yield of 49% and a purification factor of 1.472 without the addition of surfactants or pH adjustment. The most effective ATPSystem consists of polyethylene glycol 6000 and phosphate salt with a recovery yield of 97%, partitioning coefficient of about 28 and purification factor of 1.466. Ultrafiltration gave a recovery yield of 92% and purification factor of 1.123. Two possible ways of C-PC purification depending on the crude extract purity and concentration was proposed.

高效的分离纯化方法是生物制品生产和进一步利用的主要障碍。一种这样有价值的生物产品是热稳定的 C-藻蓝蛋白 (C-PC)。开发具有适当纯度的 C-PC 生产有很大的商业需求。然而，所选择的方法及其顺序也取决于从生物质中获得的粗提物的质量。在本文中，三种下游处理方法，如泡沫分馏 (FF)，据我们所知尚未用于分离 C-PC，水两相萃取 (ATPE) 和常规超滤用于纯化从聚球藻的生物质中获得的蛋白质浓度和纯度越来越低的两种粗提取物的热稳定 C-PC. PCC6715。FF 工艺的回收率为 49%，纯化系数为 1.472，无需添加表面活性剂或调节 pH 值。最有效的ATP系统由聚乙二醇6000和磷酸盐组成，回收率为97%，分配系数约为28，净化因子为1.466。超滤回收率为 92%，纯化系数为 1.123。根据粗提物纯度和浓度，提出了两种可能的 C-PC 纯化方法。