当前位置: X-MOL 学术J. Mol. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Single Molecule RNA Localization and Translation in the Mammalian Oocyte and Embryo
Journal of Molecular Biology ( IF 4.7 ) Pub Date : 2021-07-20 , DOI: 10.1016/j.jmb.2021.167166
Denisa Jansova 1 , Daria Aleshkina 1 , Anna Jindrova 1 , Rajan Iyyappan 1 , Qin An 2 , Guoping Fan 2 , Andrej Susor 1
Affiliation  

During oocyte growth the cell accumulates RNAs to contribute to oocyte and embryo development which progresses with ceased transcription. To investigate the subcellular distribution of specific RNAs and their translation we developed a technique revealing several instances of localized translation with distinctive regulatory implications. We analyzed the localization and expression of candidate non-coding and mRNAs in the mouse oocyte and embryo. Furthermore, we established simultaneous visualization of mRNA and in situ translation events validated with polysomal occupancy. We discovered that translationally dormant and abundant mRNAs CyclinB1 and Mos are localized in the cytoplasm of the fully grown GV oocyte forming cloud-like structures with consequent abundant translation at the center of the MII oocyte. Coupling detection of the localization of specific single mRNA molecules with their translation at the subcellular context is a valuable tool to quantitatively study temporal and spatial translation of specific target mRNAs to understand molecular processes in the developing cell.



中文翻译:

哺乳动物卵母细胞和胚胎中的单分子 RNA 定位和翻译

在卵母细胞生长过程中,细胞积累 RNA 以促进卵母细胞和胚胎的发育,随着转录的停止,卵母细胞和胚胎发育。为了研究特定 RNA 的亚细胞分布及其翻译,我们开发了一种技术,揭示了具有独特监管意义的本地化翻译的几个实例。我们分析了小鼠卵母细胞和胚胎中候选非编码和 mRNA 的定位和表达。此外,我们建立了 mRNA 和原位翻译事件的同步可视化,并通过多核糖体占用进行了验证。我们发现翻译休眠和丰富的 mRNA CyclinB1Mos位于完全生长的 GV 卵母细胞的细胞质中,形成云状结构,随后在 MII 卵母细胞的中心进行大量翻译。将特定单个 mRNA 分子的定位与其在亚细胞环境中的翻译相结合,是定量研究特定目标 mRNA 的时空翻译以了解发育细胞中的分子过程的宝贵工具。

更新日期:2021-08-05
down
wechat
bug