Immunodetection on mouse routinely processed tissue via antibodies raised in mice faces cross-reactivity of the secondary anti-mouse reagents with endogenous immunoglobulins, which permeate the tissue. Various solutions to this problem have been devised and include endogenous Ig block with anti-mouse Fab fragments or directly conjugated primary antibodies. Mouse isotype-specific antibodies, differently from reagents directed against both heavy and light chains, fail to detect endogenous Ig after fixation and embedding, while providing a clean and specific detection system for mouse antibodies on mouse routinely processed tissue.

中文翻译：

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通过抗同种型二抗对小鼠组织上的小鼠抗体进行无背景检测：Mouse on Mouse Ab 染色

通过小鼠体内产生的抗体对小鼠常规处理的组织进行免疫检测面临二级抗小鼠试剂与渗透组织的内源性免疫球蛋白的交叉反应。已经设计了针对该问题的各种解决方案，包括具有抗小鼠 Fab 片段或直接偶联一抗的内源性 Ig 阻断。小鼠同种型特异性抗体与针对重链和轻链的试剂不同，在固定和包埋后无法检测内源性 Ig，同时为小鼠常规处理的组织上的小鼠抗体提供干净且特异性的检测系统。