The main phycobiliprotein (PBP) found in cyanobacteria is phycocyanin (PC). The process of recovery is based on extraction followed by a purification stage. Among the methods of PBP extraction analyzed in this work, that is, freezing and thawing under sonication bath, vortex disruption with glass beads, maceration with pestle and mortar and ultrasonic homogenizer, the most efficient method was 5 freezing cycles (− 15 ± 1 °C) for 1.5 h and thawing (25 ± 1 °C) for 30 min in an ultrasonic bath, obtaining the highest PC concentrations of 32.87 μg mL⁻¹ and 34.53 μg mL⁻¹ and purities of 0.59 and 0.55, for Anabaena variabilis and Nostoc sp., respectively. In the purification by aqueous two-phase systems (ATPS), the systems of PEG (1500 and 4000) and two salts, potassium phosphate and sodium citrate, were evaluated using different volume ratios. The best system found was PEG 1500-potassium phosphate, in the proportion PEG, 10% (w/w) and salt, 15.9% (w/w), obtaining a recovery of 82.10%, partition coefficient of 5.73 and purity of 1.12 for the extract from Anabaena variabilis and recovery of 79.10%, partition coefficient of 6.36 and purity of 1.34 for the extract from Nostoc sp., both in adequate purity conditions for food grade.

在蓝藻中发现的主要藻胆蛋白 (PBP) 是藻蓝蛋白 (PC)。回收过程基于提取和纯化阶段。在本工作分析的 PBP 提取方法中，即超声浴下冷冻和解冻、玻璃珠涡旋破碎、研杵和超声均质机浸渍，最有效的方法是 5 个冷冻循环 (- 15 ± 1 ° C) 1.5 小时，然后在超声波浴中解冻 (25 ± 1 °C) 30 分钟，获得最高的 PC 浓度 32.87 μg mL ^-1和 34.53 μg mL ^-1以及 0.59 和 0.55 的纯度，对于变异鱼腥藻和发条sp.，分别。在通过含水两相系统 (ATPS) 进行纯化时，使用不同的体积比评估了 PEG（1500 和 4000）和两种盐（磷酸钾和柠檬酸钠）的系统。发现的最佳系统是 PEG 1500-磷酸钾，比例为 PEG 10% (w/w) 和盐 15.9% (w/w)，回收率为 82.10%，分配系数为 5.73，纯度为 1.12 Anabaena variabilis的提取物和回收率为 79.10%，分配系数为 6.36 和纯度为 1.34 的Nostoc sp.提取物，两者都在食品级纯度条件下。