Pyrrolizidine alkaloids (PAs) that are plant toxin naturally produced for protection against herbivores in some plant families. They are associated with the potential hepatotoxic and carcinogenic diseases serious hepatic disease in humans and animals. As the concern of human health risk posed by exposure to PAs has been gradually increased, precise and reliable analysis is required for monitoring PAs. The present study developed a new and simple pretreatment using 50% MeOH (methanol) for quantification analysis of the PAs contained with high content in the herbal medicines. Another pretreatment method using cation-ion exchange solid-phase extraction (MCX-SPE) was employed for determining most of the PAs that are not contained in the herbal medicines. That is, the proposed LC–MS/MS method coupled with MCX-SPE extraction and 50% MeOH extraction method was developed. And to evaluate the reliability of its application for Farfarae Flos and Lithospermi Radix, a validation study was conducted. In addition, monitory study was performed with ten samples in each herbal medicine. As a result, the proposed method had good linearity with r2 ≥ 0.997. Also, the recoveries indicated to be in the ranges of 70.4–118.0% for the Farfarae Flos, 70.2–119.7% for the Lithospermi Radix. In two herbal medicines, the intra-day precision was revealed to satisfy the reference criteria in most of the PAs. In monitoring results, most of the PAs were not contained in two herbal medicines, whereas a part of PAs revealed to have high concentration in Farfarae Flos and Lithospermi Radix. The proposed method is considered as a simple and reliable method to quantify 28 PAs present in two herbal medicines. Especially, the simple MeOH extraction method seems to be available for quantification analysis of certain PAs in herbal medicines with high content.

中文翻译：

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再加上在固相萃取和MeOH萃取法测定吡咯里生物碱的LC-ESI-MS / MS分析过程款冬和紫草erythrorhzion

吡咯里西啶生物碱 (PA) 是天然产生的植物毒素，用于保护某些植物科中的食草动物。它们与人类和动物的潜在肝毒性和致癌性疾病严重肝病有关。随着对暴露于 PA 对人类健康风险的关注逐渐增加，需要对 PA 进行精确可靠的分析。本研究开发了一种新的简单的预处理方法，使用 50% MeOH（甲醇）对草药中含量高的 PA 进行定量分析。使用阳离子-离子交换固相萃取 (MCX-SPE) 的另一种预处理方法用于测定大多数不包含在草药中的 PA。那是，开发了建议的 LC-MS/MS 方法与 MCX-SPE 萃取和 50% MeOH 萃取方法相结合。并且为了评估其在 Farfarae Flos 和 Lithospermi Radix 中应用的可靠性，进行了一项验证研究。此外，对每种草药中的十个样品进行了监测研究。因此，所提出的方法具有良好的线性，r2 ≥ 0.997。此外，Farfarae Flos 的回收率范围为 70.4-118.0%，紫草为 70.2-119.7%。在两种草药中，发现日内精密度满足大多数 PA 的参考标准。在监测结果中，大部分 PAs 均不含在两种草药中，而部分 PAs 显示在 Farfarae Flos 和 Lithospermi Radix 中具有高浓度。所提出的方法被认为是一种简单可靠的方法，可以量化两种草药中存在的 28 种 PA。特别是，简单的甲醇提取方法似乎可用于高含量草药中某些 PA 的定量分析。