Puccinia Non-Expresser of Pathogenesis interactor Protein (PNPi) is a virulent protein secreted by Puccinia striiformis f. sp. tritici (Pst), causative agent of yellow stripe rust disease (YSRD) of wheat. Pst PNPi protein interacts with wheat host protein Non- Expresser of Pathogenesis Related Protein (NPR1) inside the host cell. This interaction may vary due to sequence polymorphisms in the Pst PNPi gene. In this study, we explored the sequence variation in the PNPi gene of Pst. Additionally, using bioinformatics tools, we predicted the molecular structure of PNPi and NPR1. DNA sequencing of multiple Pst samples suggested no sequence variation in screened exons. Structure of the FtsN domain of PNPi was predicted to be intrinsically disordered. However, molecular docking analysis showed that the region could be involved in establishing physical interaction with NPR1. The findings of the study are expected to further our understanding of disease biology of Pst which could further be exploited to develop diagnostic tools and/or preventive intervention.

Puccinia Non-Expresser of Pathogenesis Interactor Protein (PNPi) 是由Puccinia striiformis f分泌的一种毒性蛋白。sp. 小麦( Pst )，小麦黄条锈病 (YSRD) 的病原体。Pst PNPi 蛋白与宿主细胞内的小麦宿主蛋白非致病相关蛋白 (NPR1) 表达蛋白相互作用。由于Pst PNPi 基因中的序列多态性，这种相互作用可能会有所不同。在这项研究中，我们探索了Pst PNPi 基因的序列变异. 此外，我们使用生物信息学工具预测了 PNPi 和 NPR1 的分子结构。多个 Pst 样本的 DNA 测序表明筛选的外显子没有序列变异。预测 PNPi 的 FtsN 域的结构本质上是无序的。然而，分子对接分析表明该区域可能参与建立与 NPR1 的物理相互作用。预计该研究的结果将进一步了解Pst的疾病生物学，可以进一步利用它来开发诊断工具和/或预防干预。