Cannabidiol is a natural herbal medicine known to protect the brain from traumatic brain injury (TBI). Here, a TBI rat model was established, with cannabidiol administered intraperitoneally at doses of 5, 10, or 20 mg/kg, 30 min before surgery and 6 h after surgery until sacrifice. Brain water content, body weight, and modified neurological severity scores were determined, and enzyme-linked immunosorbent assay, immunofluorescence staining, hematoxylin and eosin staining, Nissl staining, Evans-blue dye extravasation, and western blotting were performed. Results showed that cannabidiol decreased the number of aquaporin-4-positive and glial fibrillary acidic protein-positive cells. Cannabidiol also significantly reduced the protein levels of proinflammatory cytokines (TNF-α and IL-1β) and significantly increased the expression of tight junction proteins (claudin-5 and occludin). Moreover, cannabidiol administration significantly mitigated water content in the brain after TBI and blood–brain barrier disruption and ameliorated the neurological deficit score after TBI. Cannabidiol administration improved the integrity and permeability of the blood–brain barrier and reduced edema in the brain after TBI.

大麻二酚是一种天然草药，可以保护大脑免受创伤性脑损伤 (TBI)。在这里，建立了 TBI 大鼠模型，在手术前 30 分钟和手术后 6 小时以 5、10 或 20 mg/kg 的剂量腹腔注射大麻二酚直至处死。测定脑含水量、体重和改良的神经系统严重程度评分，并进行酶联免疫吸附试验、免疫荧光染色、苏木精和伊红染色、尼氏染色、伊文思蓝染料外渗和蛋白质印迹。结果表明，大麻二酚减少了水通道蛋白4阳性和胶质纤维酸性蛋白阳性细胞的数量。Cannabidiol 还显着降低促炎细胞因子（TNF-α 和 IL-1β）的蛋白质水平，并显着增加紧密连接蛋白（claudin-5 和 occludin）的表达。此外，大麻二酚给药显着降低了 TBI 和血脑屏障破坏后大脑中的水含量，并改善了 TBI 后的神经功能缺损评分。大麻二酚给药改善了血脑屏障的完整性和通透性，并减少了 TBI 后大脑中的水肿。