Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response,Archives of Insect Biochemistry and Physiology

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Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-07-19 , DOI: 10.1002/arch.21830
Hui-Juan Guo ₁ , Li-Jun Wang ₁ , Chen Wang ₁ , De-Zheng Guo ₁ , Bao-Hua Xu ₂ , Xing-Qi Guo ₁ , Han Li ₁

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Zinc finger proteins (ZFPs) are a class of transcription factors that contain zinc finger domains and play important roles in growth, aging, and responses to abiotic and biotic stresses. These proteins activate or inhibit gene transcription by binding to single-stranded DNA or RNA and through RNA/DNA bidirectional binding and protein–protein interactions. However, few studies have focused on the oxidation resistance functions of ZFPs in insects, particularly Apis cerana. In the current study, we identified a ZFP41 gene from A. cerana, AcZFP41, and verified its function in oxidative stress responses. Real-time quantitative polymerase chain reaction showed that the transcription level of AcZFP41 was upregulated to different degrees during exposure to oxidative stress, including that induced by extreme temperature, UV radiation, or pesticides. In addition, the silencing of AcZFP41 led to changes in the expression patterns of some known antioxidant genes. Moreover, the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and glutathione S-transferase (GST) in AcZFP41-silenced honeybees were higher than those in a control group. In summary, the data indicate that AcZFP41 is involved in the oxidative stress response. The results provide a theoretical basis for further studies of zinc finger proteins and improve our understanding of the antioxidant mechanisms of honeybees.

中文翻译：

蜜蜂锌指蛋白41基因的鉴定及其在氧化应激反应中的作用

锌指蛋白 (ZFP) 是一类含有锌指结构域的转录因子，在生长、衰老以及对非生物和生物胁迫的反应中起重要作用。这些蛋白质通过与单链 DNA 或 RNA 结合以及通过 RNA/DNA 双向结合和蛋白质-蛋白质相互作用来激活或抑制基因转录。然而，很少有研究关注 ZFPs 在昆虫中的抗氧化功能，尤其是蜜蜂。在目前的研究中，我们从A. cerana 中鉴定了一个 ZFP41 基因AcZFP41，并验证了其在氧化应激反应中的功能。实时定量聚合酶链反应表明AcZFP41的转录水平在暴露于氧化应激（包括由极端温度、紫外线辐射或杀虫剂引起的氧化应激）期间不同程度地上调。此外，AcZFP41的沉默导致一些已知抗氧化基因的表达模式发生变化。此外，AcZFP41沉默蜜蜂的抗氧化酶过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、过氧化物酶 (POD) 和谷胱甘肽S-转移酶 (GST) 的活性高于对照组。总之，数据表明AcZFP41参与氧化应激反应。该结果为进一步研究锌指蛋白提供了理论依据，提高了我们对蜜蜂抗氧化机制的理解。

更新日期：2021-08-17

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