Silencing of a putative alanine aminotransferase (ALT) gene influences free amino acid composition in hemolymph and fecundity of the predatory bug, Cyrtorhinus lividipennis Reuter,Archives of Insect Biochemistry and Physiology

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Silencing of a putative alanine aminotransferase (ALT) gene influences free amino acid composition in hemolymph and fecundity of the predatory bug, Cyrtorhinus lividipennis Reuter
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-07-19 , DOI: 10.1002/arch.21836
Sheraz Ahmad ₁ , Lu Jiang ₁ , Sui Zheng ₁ , Yu Chen ₁ , Jie Y Zhang ₁ , David Stanley ₂ , Hong Miao ₃ , Lin Q Ge ₁

Affiliation

In Asian rice systems, Cyrtorhinus lividipennis Reuter is an important predator that preys on rice planthopper eggs and young nymphs, as a primary food source. Alanine aminotransferase (ALT) acts in many physiological and biochemical processes in insects. We cloned the full-length complementary DNA of C. lividipennis ClALT. Expression analysis showed higher expression in the fat body and midgut compared to other tissues. It is expressed in all C. lividipennis developmental stages and at least four organs. Silencing of ClALT by RNA interference significantly decreased the ClALT enzyme activity and ClALT expression compared to dsGFP-treated controls at 2 days after emergence (DAE). Silencing of ClALT influenced free hemolymph amino acid compositions, resulting in a reduction of Aspartic acid (Asp) and Alanine (Ala) proportions, and increased Cysteine (Cys) and Valine (Val) proportions in females at 2 DAE. dsClALT treatments led to decreased soluble total protein concentrations in ovary and fat body, and to lower reduced vitellogenin (Vg) expression, body weight, and the numbers of laid eggs. The double-stranded RNA viruse treatments also led to prolonged preoviposition periods and hindered ovarian development. Western blot analysis indicated that silencing ClALT also led to reduced fat body Vg protein abundance at 2 DAE. These data support our hypothesis that ClALT influences amino acid metabolism and fecundity in C. lividipennis.

中文翻译：

假定的丙氨酸氨基转移酶 (ALT) 基因的沉默影响血淋巴中的游离氨基酸组成和捕食性昆虫的繁殖力，Cyrtorhinus lividipennis Reuter

在亚洲水稻系统中，Cyrtorhinus lividipennis Reuter 是一种重要的捕食者，以稻飞虱卵和幼虫为主要食物来源。丙氨酸氨基转移酶 (ALT) 在昆虫的许多生理和生化过程中起作用。我们克隆了C. lividipennis ClALT的全长互补 DNA 。表达分析显示与其他组织相比，脂肪体和中肠中的表达更高。它在所有C. lividipennis发育阶段和至少四个器官中表达。在出现后 2 天 (DAE)，与 dsGFP 处理的对照相比，通过 RNA 干扰沉默ClALT显着降低了 ClALT 酶活性和ClALT表达。沉默的ClALT影响游离血淋巴氨基酸组成，导致女性在 2 DAE 时天冬氨酸 (Asp) 和丙氨酸 (Ala) 比例降低，半胱氨酸 (Cys) 和缬氨酸 (Val) 比例增加。ds ClALT处理导致卵巢和脂肪体中可溶性总蛋白浓度降低，并降低降低的卵黄蛋白原 ( Vg ) 表达、体重和产卵数。双链RNA病毒治疗还导致产卵前期延长并阻碍卵巢发育。蛋白质印迹分析表明，沉默ClALT还导致在 2 DAE 时脂肪体 Vg 蛋白丰度降低。这些数据支持我们的假设，即 ClALT 影响氨基酸代谢和繁殖力C. lividipennis。

更新日期：2021-09-20

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