A genome-wide identification and analysis of the homeobox genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae),Archives of Insect Biochemistry and Physiology

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A genome-wide identification and analysis of the homeobox genes in the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-07-19 , DOI: 10.1002/arch.21833
Sheng-Jie Fu ₁ , Jin-Li Zhang ₁ , Hai-Jun Xu _{1,

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Affiliation

The homeobox family is a large and diverse superclass of genes, many of which act as transcription factors that play important roles in tissue differentiation and embryogenesis in animals. The brown planthopper (BPH), Nilaparvata lugens, is the most destructive pest of rice in Asia, and high fecundity contributes significantly to its ecological success in natural and agricultural habits. Here, we identified 94 homeobox genes in BPH, which could be divided into 75 gene families and 9 classes. This number is comparable to the number of homeobox genes found in the honeybee Apis mellifera, but is slightly less than in Drosophila or the red flour beetle Tribolium castaneum. A spatio-temporal analysis indicated that most BPH homeobox genes were expressed in a development and tissue-specific manner, of which 21 genes were highly expressed in ovaries. RNA interference (RNAi)-mediated functional assay showed that 22 homeobox genes were important for nymph development and the nymph to adult transition, whereas 67 genes were dispensable during this process. Fecundity assay showed that knockdown of 13 ovary-biased genes (zfh1, schlank, abd-A, Lim3_2, Lmxb, Prop, ap_1, Not, lab, Hmx, vis, Pknox, and C15) led to the reproductive defect. This is the first comprehensive investigation into homeobox genes in a hemipteran insect and thus helps us to understand the functional significance of homeobox genes in insect reproduction.

中文翻译：

褐飞虱 Nilaparvata lugens (Hemiptera: Delphacidae) 同源框基因的全基因组鉴定和分析

同源盒家族是一个庞大而多样的基因超类，其中许多作为转录因子在动物的组织分化和胚胎发生中发挥重要作用。褐飞虱 (BPH) Nilaparvata lugens是亚洲最具破坏性的水稻害虫，高繁殖力对其自然和农业习惯的生态成功做出了重大贡献。在这里，我们在 BPH 中鉴定了 94 个同源框基因，可分为 75 个基因家族和 9 个类别。这个数字与蜜蜂Apis mellifera中发现的同源框基因的数量相当，但略低于果蝇或红粉甲虫Tribolium castaneum。. 时空分析表明，大多数 BPH 同源盒基因以发育和组织特异性方式表达，其中 21 个基因在卵巢中高表达。RNA 干扰 (RNAi) 介导的功能测定表明，22 个同源框基因对若虫发育和若虫向成虫过渡很重要，而 67 个基因在此过程中是可有可无的。生育力测定表明，13 个卵巢偏向基因（zfh1、schlank、abd-A、Lim3_2、Lmxb、Prop、ap_1、Not、lab、Hmx、vis、Pknox和C15 )导致生殖缺陷。这是对半翅目昆虫同源框基因的首次综合研究，有助于我们了解同源框基因在昆虫繁殖中的功能意义。

更新日期：2021-08-17

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