Oryzaephilus surinamensis, a common grain product pest around the world, particularly causing great damage and losses to the date crops available in the storage. It has been traditionally resisted by using organic pesticides despite suspicions of their harmful effects on dates. This study aimed at the evaluation of the usage of ozone gas as an effective pesticide and also focuses on the safety alternatives provided by the respective method as compared to the usage of phosphine as a pesticide. While studying the ozone effects, the O. surinamensis was exposed to the gas for a variety of time scales (2, 4 and 6 h), distinctly for every life stages of the insect such as egg, larva and adults, infested the Phoenix dactylifera L date palm species present in the Siwa oasis. The obtained results showed that the adult insects had high resistance to ozone gas, while the larvae and egg stages were less resistant. The reduction rate of vital insect was 100% in all stages. Start codon analysis of DNA also showed that there are some SCoT primers can be identified and differentiated between the different treatments and control. On the other hand, the percentage of polymorphisms in insects was 50% while in date kernels 25% by six SCoT primers used. This study highlighted higher efficacy and safety regarding the usage of ozone gas in effectively controlling the manifestation of O. Surinamensis and thereby reducing the loss of stored date crops as compared to the phosphine.

Oryzaephilus surinamensis，一种世界范围内常见的粮食产品害虫，特别是对仓库中可用的枣类作物造成巨大破坏和损失。尽管怀疑它们对枣有有害影响，但传统上一直通过使用有机杀虫剂来抵制它。本研究旨在评估臭氧气体作为有效杀虫剂的使用情况，并重点关注与磷化氢作为杀虫剂的使用相比，各自方法提供的安全替代方案。在研究臭氧效应时，O. surinamensis暴露在气体中的时间跨度不同（2、4 和 6 小时），尤其是在昆虫的每个生命阶段，例如卵、幼虫和成虫，都感染了Phoenix dactylifera锡瓦绿洲中存在的 L 枣椰树种。所得结果表明，成虫对臭氧气体具有较高的抵抗力，而幼虫和卵阶段的抵抗力较差。各阶段活虫减少率为100%。DNA 的起始密码子分析还表明，有一些 SCoT 引物可以在不同处理和对照之间进行鉴定和区分。另一方面，昆虫的多态性百分比为 50%，而枣核中使用的六种 SCoT 引物的多态性百分比为 25%。与磷化氢相比，这项研究强调了使用臭氧气体在有效控制O. Surinamensis的表现方面具有更高的功效和安全性，从而减少了贮藏作物的损失。