International Dairy Journal ( IF 3.1 ) Pub Date : 2021-07-20 , DOI: 10.1016/j.idairyj.2021.105147 Jelena Zagorska 1 , Ruta Galoburda 1 , Svetlana Raita 2 , Marika Liepa 1
High pressure treatments at 400, 500, 550, and 600 MPa for 15–30 min at room temperature were applied to milk inoculated with a single strain of Gram-positive (Listeria monocytogenes and Staphylococcus aureus) or Gram-negative (Escherichia coli) bacteria or their mixture. This study showed that the high pressure-induced inactivation of microorganisms depends on such factors as microbial species, pressure, concentration of the inoculant, and presence of other bacterial species. There were significant (p < 0.05) differences in the inactivation rate of bacteria among pressure treatments. E. coli ATCC 25922 showed lower pressure resistance than L. monocytogenes ATCC 7644 and S. aureus ATCC 25923. S. aureus is more resistant in a mixture; recovery of S. aureus was detected after 8 days for a single inoculum and 1 day in a mixture of milk storage at 4 ± 1 °C.
中文翻译:
高压处理后牛奶中单独和混合的细菌菌株的灭活和回收
在室温下以 400、500、550 和 600 MPa 高压处理 15-30 分钟,用于接种单株革兰氏阳性菌(单核细胞增生李斯特菌和金黄色葡萄球菌)或革兰氏阴性菌(大肠杆菌)的牛奶或它们的混合物。该研究表明,高压诱导的微生物灭活取决于微生物种类、压力、接种剂浓度以及其他细菌种类的存在等因素。 压力处理之间细菌的灭活率存在显着差异(p < 0.05)。大肠杆菌ATCC 25922 的耐压性低于单核细胞增生李斯特菌ATCC 7644 和金黄色葡萄球菌ATCC 25923 。金黄色葡萄球菌在混合物中更具抵抗力;单次接种 8 天后检测到金黄色葡萄球菌的恢复,在 4 ± 1 °C 的牛奶混合物中储存 1 天后检测到金黄色葡萄球菌的恢复。