ABSTRACT

Management of broomrape (Orobanche crenata Fosk.) that causes important damages on lentil production becomes a veritable concern in the Mediterranean region. Eighty lentil accessions were evaluated for resistance to O. crenata under field and controlled conditions. Both genotypes ILL6415 and ILL7723 expressed the highest resistance level under field and pot experiment with low Orobanche infestation and relatively high seed yield (50.1 g m⁻²). Such resistance was associated with physiological and biochemical changes in metabolites profiling. In total, 109 and 115 metabolites were identified in the lipophilic phase of both ILL6415 and ILL7723, respectively, against only 92 metabolites recorded for susceptible check Zaaria. Significant differences were observed in metabolite concentrations (fatty acids, sterols alkanes) between roots and shoots of susceptible and resistant infested plants. Accumulation of α-linolenic acid and arachidic acid was more pronounced in the resistant genotypes ILL6415, ILL7723 which could be associated with resistance pathways involved in the resistance to O. crenata.

摘要

对小扁豆生产造成重大损害的扫帚菜( Orobanche crenata Fosk. ) 的管理成为地中海地区一个名副其实的问题。在田间和受控条件下评估了 80 个小扁豆种质对O. crenata 的抗性。ILL6415 和 ILL7723 基因型在田间和盆栽试验中均表现出最高的抗性水平，Orobanche侵染率低，种子产量相对较高（50.1 g m ^-2）。这种抗性与代谢物分析的生理和生化变化有关。总共在 ILL6415 和 ILL7723 的亲脂相中分别鉴定了 109 和 115 种代谢物，而仅针对易感检查 Zaaria 记录的 92 种代谢物。在易感和抗性侵染植物的根和芽之间观察到代谢物浓度（脂肪酸、甾醇烷烃）的显着差异。抗性基因型 ILL6415、ILL7723 中 α-亚麻酸和花生酸的积累更为明显，这可能与O. crenata抗性中涉及的抗性途径有关。