Serum interleukin- (IL-) 27 level has been reported to increase in patients with several autoimmune diseases; however, its significance in patients with antineutrophil cytoplasmic antibody- (ANCA-) associated vasculitis (AAV) is unknown. In this study, we investigated the associations between serum IL-27, laboratory features, and activity of AAV and evaluate the predictive ability of serum IL-27 level for disease activity. This study included 77 AAV patients, and we collected clinical and laboratory data at blood sampling. Inflammation-related variables included white blood cell, neutrophil, lymphocyte and platelet counts, serum albumin, erythrocyte sedimentation rate, and C-reactive protein levels. Serum IL-27 and IL-18 levels were measured from stored sera using Human Magnetic Luminex® assay. High disease activity of AAV was defined as the highest tertile of Birmingham vasculitis activity score (BVAS) (≥11). The mean age of the enrolled patients was 59.9 years, and 38 (49.4%) were diagnosed as microscopic polyangiitis. In the multivariable analysis, serum albumin () and serum IL-27 level () were significantly associated with BVAS. Furthermore, patients with renal manifestation exhibited higher serum IL-27 (mean 308.7 pg/mL vs. 105.8 pg/mL) and IL-18 levels (mean 376.7 pg/mL vs. 270.4 pg/mL) than those without. On applying the optimal cut-off of serum IL-27 level for predicting high activity, AAV patients with serum  pg/mL had a significantly higher risk for having high disease activity than those with serum  pg/mL (relative risk 3.380, 95% confidence interval 1.223, 9.345, ). These results suggest that serum IL-27 level is associated with the cross-sectional activity and the presence of renal manifestation and could be used to predict high disease activity in patients with AAV.

中文翻译：

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血清 IL-27 水平对评估抗中性粒细胞胞浆抗体相关性血管炎活性的预测能力

据报道，患有多种自身免疫性疾病的患者的血清白细胞介素 (IL-) 27 水平会升高；然而，它在抗中性粒细胞胞浆抗体（ANCA-）相关血管炎（AAV）患者中的意义尚不清楚。在这项研究中，我们调查了血清 IL-27、实验室特征和 AAV 活性之间的关联，并评估了血清 IL-27 水平对疾病活动的预测能力。这项研究包括 77 名 AAV 患者，我们在采血时收集了临床和实验室数据。炎症相关变量包括白细胞、中性粒细胞、淋巴细胞和血小板计数、血清白蛋白、红细胞沉降率和 C 反应蛋白水平。使用 Human Magnetic Luminex® 测定法从储存的血清中测量血清 IL-27 和 IL-18 水平。AAV 的高疾病活动性被定义为伯明翰血管炎活动性评分 (BVAS) (≥11) 的最高三分位数。入组患者的平均年龄为 59.9 岁，其中 38 例（49.4%）被诊断为镜下多血管炎。在多变量分析中，血清白蛋白（)和血清 IL-27 水平 ()与 BVAS 显着相关。此外，有肾脏表现的患者的血清 IL-27（平均 308.7 pg/mL 对 105.8 pg/mL）和 IL-18 水平（平均 376.7 pg/mL 对 270.4 pg/mL）比没有的患者更高。应用血清 IL-27 水平的最佳截止值预测高活性、血清 AAV 患者 pg/mL 具有高疾病活动性的风险显着高于血清 pg/mL（相对风险 3.380、95% 置信区间 1.223、9.345、）。这些结果表明，血清 IL-27 水平与横断面活动和肾脏表现的存在相关，可用于预测 AAV 患者的高疾病活动性。