This work reports, the obtainment and stabilization of organic nanoparticles (γ-sitosterol) and Ag cations on the external area of Ca-clinoptilolite zeolite (Z17) are presented. The novelty of the present study lies in the formation and stabilization of nanoparticle organic molecules (γ-sitosterol) and inorganic cations (Ag) on the external area of Ca-clinoptilolite zeolite. The γ-sitosterol nanoparticles were obtained using Tournefortia hirsutissima and were later stabilized on the external area of Ca-clinoptilolite. The Ca-clinoptilolite-rich mineral as well as silver zeolitic minerals was characterized by using X-ray diffraction (XRD) and scanning electron microscopy (SEM); elementary composition of the zeolitic mineral was determined by microanalyses (EDS) and high-resolution adsorption (HRADS) using N₂ adsorption as a probe molecule. The EDS results about the exchange with Ag indicate the following sequence: Z17Ag03 > Z17Ag-09 > Z17Ag-06 > Z17Ag-01. The XRD studies indicate that only in the sample Z17Ag-03 there is an average crystal size of nanoparticles formed of 45.387 nm as determined by Sherrer’s equation. SEM images of clinoptilolite showed the presence of crystals with irregular and poorly defined box shapes that are typical for this type of zeolites. The nanoporosity of natural zeolites was studied by N₂ adsorption; external areas were estimated through the De Boer and BET equations. The specific surface area calculated by BET equation, , presents the following sequence: γ-SZ17 > Z17 > Z17Ag09 > Z17Ag03 > Z17Ag01 > Z17Ag06, while for the values of the external area obtained by the method, the sequence is : γ-Z17 > Z17 > Z17Ag03 > z17Ag09 > Z17Ag06 > Z17Ag01. For its part, the pore size distributions tell us that the sample Z17 exhibits a sharp multimodal distribution with pore size maxima happening at 1.262, 1.933, 3.824, and 4.282 nm. Z17Ag01 exhibits a bimodal distribution with pore size maxima occurring at 5.45 and 5.533 nm. The sample Z17Ag06 exhibits a sharp bimodal distribution with pore size maxima happening at 3.666 and 7.021 nm, and the sample Z17Ag09 exhibits a sharp trimodal distribution with pore size maxima happening at 1.853, 4.275 and 5.967 nm. Antibacterial activities of the exchanged samples with Ag on the external surface area were measured as a function of exchange level against Gram-positive bacteria (P. aureoginosa, K. pneumonie, and A. baumannii) and Gram-negative bacteria (E. coli, S. saprophyticcus, S. aureus, and E. feacalis); the selectivity sequence of the clinoptilolite and antibacterial activity results are presented. Z17Ag09 clinoptilolite seemed to be a promising antibacterial material.

中文翻译：

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斜发沸石沸石上γ-谷甾醇和银的纳米颗粒

这项工作报告，介绍了有机纳米粒子（γ-谷甾醇）和银阳离子在Ca-斜发沸石沸石（Z17）的外部区域的获得和稳定。本研究的新颖之处在于纳米颗粒有机分子 ( γ-谷甾醇) 和无机阳离子 (Ag) 在 Ca-斜发沸石沸石的外部区域的形成和稳定化。的γ谷甾醇的纳米颗粒使用得到紫丹属hirsutissima并且后来稳定在Ca-斜发沸石的外部区域。使用X射线衍射（XRD）和扫描电子显微镜（SEM）对富含Ca的斜发沸石矿物和银沸石矿物进行了表征；沸石矿物的元素组成通过微量分析 (EDS) 和高分辨率吸附 (HRADS) 使用 N ₂吸附作为探针分子。与Ag交换的EDS结果表明以下顺序：Z17Ag03>Z17Ag-09>Z17Ag-06>Z17Ag-01。XRD 研究表明，仅在样品 Z17Ag-03 中，形成的纳米颗粒的平均晶体尺寸为 45.387 nm，由 Sherrer 方程确定。斜发沸石的 SEM 图像显示存在具有不规则和边界不清的盒子形状的晶体，这是此类沸石的典型特征。通过N ₂吸附研究天然沸石的纳米孔隙率；外部面积是通过 De Boer 和 BET 方程估计的。由 BET 方程计算的比表面积, ,呈现以下序列: γ-SZ17 > Z17 > Z17Ag09 > Z17Ag03 > Z17Ag01 > Z17Ag06，而该方法得到的外面积值的顺序为：γ -Z17 > Z17 > Z17Ag03 > z17Ag09 > Z17Ag06 > Z17Ag01。就其本身而言，孔径分布告诉我们样品 Z17 表现出尖锐的多峰分布，孔径最大值发生在 1.262、1.933、3.824 和 4.282 nm。Z17Ag01 呈现双峰分布，孔径最大值出现在 5.45 和 5.533 nm。样品 Z17Ag06 表现出明显的双峰分布，孔径最大值出现在 3.666 和 7.021 nm，而样品 Z17Ag09 表现出明显的三峰分布，孔径最大值出现在 1.853、4.275 和 5.967 nm。有Ag上的外表面面积的交换的样品的抗菌活性测量为交换程度的抗革兰氏阳性菌的功能（P. aureoginosa，K. pneumonie，和鲍曼不动杆菌）和革兰氏阴性细菌（大肠杆菌，S. saprophyticcus，金黄色葡萄球菌，和E. feacalis）; 给出了斜发沸石的选择性序列和抗菌活性结果。Z17Ag09 斜发沸石似乎是一种很有前途的抗菌材料。