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lncRNA TSPEAR-AS2, a Novel Prognostic Biomarker, Promotes Oral Squamous Cell Carcinoma Progression by Upregulating PPM1A via Sponging miR-487a-3p
Disease Markers Pub Date : 2021-07-19 , DOI: 10.1155/2021/2217663
Yi-Chao Xia 1 , Jun Cao 1 , Jing Yang 1 , Ying Zhang 1 , Yong-Sheng Li 1
Affiliation  

Background. Long noncoding RNA (lncRNA) critically impacts the modulation of tumor developments and progressions. Our study is aimed at investigating the expressing patterns, clinical significance, and biological roles of lncRNA TSPEAR-AS2 (TSPEAR-AS2) in oral squamous cell carcinoma (OSCC). Material and Approach. The expressing states achieved by TSPEAR-AS2 were examined in OSCC specimens and cell lines by RT-PCR. The clinical significance of TSPEAR-AS2 was statistically analyzed. OSCC proliferating, invading, and migrating processes were examined with the use of wound healing assays, transwell, colony formation, and cell counting kit-8. Additionally, the downstream molecular mechanism of TSPEAR-AS2 in OSCC was explored. Results. TSPEAR-AS2 was overexpressed in OSCC tumors and cells. High TSPEAR-AS2 was associated with advanced TNM stage. Patients with high TSPEAR-AS2 expression displayed a shorter disease-free survival and total survival of OSCC patients than those with low TSPEAR-AS2 expressing level. It was found that knockdown of TSPEAR-AS2 could inhibit the proliferating, invading, and migrating processes pertaining to OSCC cells. Luciferase reporter tests and RNA pull-down results revealed that TSPEAR-AS2 enhanced the expressions of PPM1A by regulating miR-487a-3p, and TSPEAR-AS2 could be adopted as a miR-487a-3p sponge to inhibit PPM1A expression. Conclusion. Our study highlighted the significance of the TSPEAR-AS2/miR-487a-3p/PPM1A axis within OSCC progression and offered a novel biomarker and novel strategies for OSCC treatments.

中文翻译:

lncRNA TSPEAR-AS2,一种新型预后生物标志物,通过海绵化 miR-487a-3p 上调 PPM1A 促进口腔鳞状细胞癌进展

背景。长链非编码 RNA (lncRNA) 严重影响肿瘤发展和进展的调节。我们的研究旨在调查 lncRNA TSPEAR-AS2 (TSPEAR-AS2) 在口腔鳞状细胞癌 (OSCC) 中的表达模式、临床意义和生物学作用。材料和方法。通过 RT-PCR 在 OSCC 标本和细胞系中检查 TSPEAR-AS2 实现的表达状态。对TSPEAR-AS2的临床意义进行统计学分析。使用伤口愈合测定、transwell、集落形成和细胞计数 kit-8 检查 OSCC 的增殖、入侵和迁移过程。此外,还探讨了 TSPEAR-AS2 在 OSCC 中的下游分子机制。结果. TSPEAR-AS2 在 OSCC 肿瘤和细胞中过表达。高 TSPEAR-AS2 与晚期 TNM 阶段相关。与低 TSPEAR-AS2 表达水平的患者相比,具有高 TSPEAR-AS2 表达水平的患者表现出更短的 OSCC 患者的无病生存期和总生存期。发现 TSPEAR-AS2 的敲低可以抑制与 OSCC 细胞有关的增殖、入侵和迁移过程。荧光素酶报告基因检测和RNA pull-down结果显示,TSPEAR-AS2通过调节miR-487a-3p增强PPM1A的表达,TSPEAR-AS2可作为miR-487a-3p海绵抑制PPM1A的表达。结论. 我们的研究强调了 TSPEAR-AS2/miR-487a-3p/PPM1A 轴在 OSCC 进展中的重要性,并为 OSCC 治疗提供了新的生物标志物和新策略。
更新日期:2021-07-19
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