Background. Long noncoding RNA (lncRNA) critically impacts the modulation of tumor developments and progressions. Our study is aimed at investigating the expressing patterns, clinical significance, and biological roles of lncRNA TSPEAR-AS2 (TSPEAR-AS2) in oral squamous cell carcinoma (OSCC). Material and Approach. The expressing states achieved by TSPEAR-AS2 were examined in OSCC specimens and cell lines by RT-PCR. The clinical significance of TSPEAR-AS2 was statistically analyzed. OSCC proliferating, invading, and migrating processes were examined with the use of wound healing assays, transwell, colony formation, and cell counting kit-8. Additionally, the downstream molecular mechanism of TSPEAR-AS2 in OSCC was explored. Results. TSPEAR-AS2 was overexpressed in OSCC tumors and cells. High TSPEAR-AS2 was associated with advanced TNM stage. Patients with high TSPEAR-AS2 expression displayed a shorter disease-free survival and total survival of OSCC patients than those with low TSPEAR-AS2 expressing level. It was found that knockdown of TSPEAR-AS2 could inhibit the proliferating, invading, and migrating processes pertaining to OSCC cells. Luciferase reporter tests and RNA pull-down results revealed that TSPEAR-AS2 enhanced the expressions of PPM1A by regulating miR-487a-3p, and TSPEAR-AS2 could be adopted as a miR-487a-3p sponge to inhibit PPM1A expression. Conclusion. Our study highlighted the significance of the TSPEAR-AS2/miR-487a-3p/PPM1A axis within OSCC progression and offered a novel biomarker and novel strategies for OSCC treatments.

中文翻译：

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lncRNA TSPEAR-AS2，一种新型预后生物标志物，通过海绵化 miR-487a-3p 上调 PPM1A 促进口腔鳞状细胞癌进展

背景。长链非编码 RNA (lncRNA) 严重影响肿瘤发展和进展的调节。我们的研究旨在调查 lncRNA TSPEAR-AS2 (TSPEAR-AS2) 在口腔鳞状细胞癌 (OSCC) 中的表达模式、临床意义和生物学作用。材料和方法。通过 RT-PCR 在 OSCC 标本和细胞系中检查 TSPEAR-AS2 实现的表达状态。对TSPEAR-AS2的临床意义进行统计学分析。使用伤口愈合测定、transwell、集落形成和细胞计数 kit-8 检查 OSCC 的增殖、入侵和迁移过程。此外，还探讨了 TSPEAR-AS2 在 OSCC 中的下游分子机制。结果. TSPEAR-AS2 在 OSCC 肿瘤和细胞中过表达。高 TSPEAR-AS2 与晚期 TNM 阶段相关。与低 TSPEAR-AS2 表达水平的患者相比，具有高 TSPEAR-AS2 表达水平的患者表现出更短的 OSCC 患者的无病生存期和总生存期。发现 TSPEAR-AS2 的敲低可以抑制与 OSCC 细胞有关的增殖、入侵和迁移过程。荧光素酶报告基因检测和RNA pull-down结果显示，TSPEAR-AS2通过调节miR-487a-3p增强PPM1A的表达，TSPEAR-AS2可作为miR-487a-3p海绵抑制PPM1A的表达。结论. 我们的研究强调了 TSPEAR-AS2/miR-487a-3p/PPM1A 轴在 OSCC 进展中的重要性，并为 OSCC 治疗提供了新的生物标志物和新策略。