Introduction

Cystic echinococcosis is a globally distributed zoonotic disease of great medical and veterinary importance, which is caused by the cestode Echinococcus granulosus sensu lato. In Ukraine, two areas of the prominent circulation of the parasite are established, the southern steppe zone with sheep as the main transmitter, and the northern forest-steppe zone and Polissia, where pigs are mainly responsible for maintaining the E. granulosus transmission.

Methods

Given that only a few studies have so far addressed the genetic diversity of the parasite in Ukraine, we have sequenced partial mitochondrial genes of cytochrome c oxidase 1 (789 bp), NADH dehydrogenase 1 (602 bp) and 12S rRNA (333–334 bp) in pig metacestodes from the Sumy region (farms close to Sumy, northeastern Ukraine) and the Kyiv region (a farm in Bila Tserkva, central Ukraine).

Results

Four isolates from four pigs in the Sumy region were identified as E. canadensis (G7 genotype), the major E. granulosus s.l. species circulating in Eastern Europe, including the three microvariants (G7A, G7B, G7C). Three isolates from the two pigs in the Kyiv region were classified as E. granulosus s.s. (G1 genotype), including one microvariant (G1A).

Conclusion

To our knowledge, this is the first genetic record of E. granulosus s.s. with the presumed highest infectivity and virulence among the E. granulosus s.l. species in Ukraine. The finding has implications for public health as local control programmes should take into consideration different development rate of this parasite in dogs and the greater risk of the species for human infection.

中文翻译：

---

乌克兰细粒棘球绦虫分子研究及国内首例细粒棘球绦虫（G1基因型）基因鉴定

介绍

囊性棘球蚴病是一种全球分布的人畜共患病，具有重要的医学和兽医重要性，由细粒棘球绦虫引起。在乌克兰，建立了寄生虫传播显着的两个区域，以羊为主要传播者的南部草原区和北部森林草原区和波利西亚，猪主要负责维持细粒沙门氏菌的传播。

方法

鉴于迄今为止只有少数研究解决了乌克兰寄生虫的遗传多样性，我们对细胞色素c氧化酶 1 (789 bp)、NADH 脱氢酶 1 (602 bp) 和 12S rRNA (333-334 bp)的部分线粒体基因进行了测序）在来自苏梅地区（乌克兰东北部苏梅附近的农场）和基辅地区（乌克兰中部比拉采尔瓦的一个农场）的猪后节。

结果

Sumy 地区四头猪的四个分离株被鉴定为加拿大大肠杆菌（G7 基因型），这是在东欧流行的主要细粒大肠杆菌 sl物种，包括三个微变体（G7A、G7B、G7C）。基辅地区两只猪的三个分离株被归类为细粒棘球绦虫 ss（G1 基因型），包括一个微变体（G1A）。

结论

据我们所知，这是E. granulosus ss的第一个遗传记录。在乌克兰的细粒棘球绦虫 sl物种中，假定的传染性和毒力最高。这一发现对公共卫生有影响，因为地方控制计划应考虑这种寄生虫在狗体内的不同发展速度以及该物种对人类感染的更大风险。