In this study, we aimed at the green synthesis of silver nanoparticles (AgNPs) using Lavandula angustifolia extract and the investigation of the anti-proliferative and apoptotic inducing effects of these nanoparticles in the U87MG glioblastoma cancer cell line. Green synthesized silver nanoparticles were characterized by various analytical techniques such as UV–Visible Spectrophotometer (UV–Vis), scanning electron microscopy (SEM) and Energy Dispersive X-ray (EDX). UV–Vis spectroscopy displayed a specific silver plasmon peak at 430 nm. U87MG cells were treated at increased concentrations with Lavandula angustifolia-AgNPs (La-AgNPs) (0–20 µg/mL) for 72 h and the anti-proliferative effects of green synthesized silver nanoparticles on U87MG cells were evaluated by MTT assay. The La- AgNPs induced a statistically significant dose-dependent decrease in proliferation and increased cytotoxicity in U87MG cells. The IC50 value is 7.536 µg/mL. Furthermore, the expression of apoptosis proteins caspase-3, caspase-8 and caspase-9 was analyzed using ELISA and caspase-3 and p53 using western blotting. The results suggest that La-AgNPs induce cell death in U87MG cells through the p53 mediated intrinsic apoptotic pathway. Together, the present findings suggest that La-AgNPs could be considered as a potential option for the treatment of glioblastoma.

在本研究中，我们旨在使用薰衣草提取物绿色合成银纳米粒子 (AgNPs) ，并研究这些纳米粒子在 U87MG 胶质母细胞瘤癌细胞系中的抗增殖和诱导凋亡作用。绿色合成的银纳米粒子通过各种分析技术进行表征，例如紫外-可见分光光度计 (UV-Vis)、扫描电子显微镜 (SEM) 和能量色散 X 射线 (EDX)。UV-Vis 光谱在 430 nm 处显示出特定的银等离子体峰。U87MG 细胞用薰衣草以增加的浓度处理-AgNPs (La-AgNPs) (0–20 µg/mL) 72 h 和绿色合成银纳米粒子对 U87MG 细胞的抗增殖作用通过 MTT 法进行评估。La-AgNPs 在 U87MG 细胞中诱导了具有统计学意义的剂量依赖性增殖减少和细胞毒性增加。IC50 值为 7.536 µg/mL。此外，使用 ELISA 分析凋亡蛋白 caspase-3、caspase-8 和 caspase-9 的表达，并使用蛋白质印迹分析 caspase-3 和 p53。结果表明，La-AgNPs 通过 p53 介导的内在凋亡途径诱导 U87MG 细胞中的细胞死亡。总之，目前的研究结果表明，La-AgNPs 可被视为治疗胶质母细胞瘤的潜在选择。