This study comprehensively presents the relationship between the bioactive substance of 70% (v/v) aqueous ethanol extract of 38 species of seaweeds (SWEs), and anti-glycation activities. The contents of bioactive substance of SWEs, such as total phenolic, total flavonoid and condensed tannins, were determined through a colorimetric analysis. Among the tested species, Ecklonia bicyclis, Ishige foliacea, and Cladophora urightiana var. minor had the highest amount of total phenolic (255.75 mg GAE/g DW), total condensed tannins (63.36 mg CE/g DW), and total flavonoid content (85.26 mg CE/g DW), respectively. Anti-glycation properties of SWEs were evaluated through advanced glycation end-products (AGEs) formation, AGEs-collagen cross-link formation, and AGEs-collagen cross-link breaking assay. Brown algae species exhibited a more prominent inhibitory activity on AGEs formation and AGEs-collagen cross-links, and the breaking of AGEs-collagen cross-links compared to that exhibited by aminoguanidine and ALT-711 (positive controls). Using principal component analysis, we confirmed that the AGEs formation inhibitory property and AGEs-collagen cross-links breaking activity were closely correlated with total phenolic and the condensed tannin contents contained in SWEs. Therefore, the bioactive substances such as phenolics and condensed tannins in seaweeds can be used as predictive indices in selecting compounds for the development of a therapeutic agent that prevents diabetic complications related to the AGEs. In addition, our results suggest that brown algae species, which contains more bioactive substances than green and red algae species, can be utilized as a promising natural resource for the prevention and alleviation of AGEs-related diabetic complications as AGE inhibitor and cross-links breaker.

本研究全面介绍了 38 种海藻 (SWE) 的 70% (v/v) 乙醇提取物的生物活性物质与抗糖化活性之间的关系。通过比色法测定SWEs的总酚、总黄酮和缩合单宁等生物活性物质的含量。在测试的物种中，Ecklonia bicyclis、Ishige foliacea和Cladophora urightiana var。次要的分别具有最高的总酚 (255.75 mg GAE/g DW)、总缩合单宁 (63.36 mg CE/g DW) 和总黄酮含量 (85.26 mg CE/g DW)。通过晚期糖基化终产物 (AGEs) 形成、AGEs-胶原交联形成和 AGEs-胶原交联断裂试验评估 SWE 的抗糖化特性。与氨基胍和ALT-711（阳性对照）相比，褐藻物种对AGEs形成和AGEs-胶原交联以及AGEs-胶原交联的破坏表现出更显着的抑制活性。通过主成分分析，我们证实AGEs形成抑制特性和AGEs-胶原交联断裂活性与SWEs中总酚和缩合单宁含量密切相关。所以，海藻中的酚类物质和缩合单宁酸等生物活性物质可用作选择化合物的预测指标，用于开发预防与 AGE 相关的糖尿病并发症的治疗剂。此外，我们的研究结果表明，褐藻物种比绿藻和红藻物种含有更多的生物活性物质，可作为 AGE 抑制剂和交联破坏剂，作为预防和缓解 AGEs 相关糖尿病并发症的有前景的自然资源。 .