Yttria-stabilized zirconia (YSZ) is being proposed as an alternative material to Titanium for dental implants due to its aesthetic and biocompatibility properties. However, is it yet to define the optimal surface treatment to improve YSZ bioactivy. Texturization is a promising approach, but the biological role of patterned YSZ surfaces in cell cultures is yet to be determined. Thus, cellular behavior of osteoblasts and fibroblasts in contact with groove-texturized YSZ surfaces was investigated. YSZ discs were groove-textured by conventional milling and Nd:YAG laser. All samples including control were sandblasted and acid-etched. Human osteoblasts and fibroblasts were cultured on discs for 14 days. Morphology and cellular adhesion were observed. Cell viability, interleukin-1β, osteopontin, collagen type I prodution, alkaline phosphatase activity, and interleukin-8 were measured. YSZ texturization by conventional milling improved osteoblasts viability and differentiation when compared to laser texturization. Fibroblasts behavior did not seem to be influenced by the texturing technique. Compared to sandblasting and acid etching currently used as gold standard for zirconia dental implants no superiority of macrotexturization was found.

中文翻译：

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氧化钇稳定氧化锆牙种植体的激光表面处理：对细胞行为的影响

由于氧化钇稳定的氧化锆 (YSZ) 具有美学和生物相容性，因此被提议作为钛的替代材料用于牙科植入物。然而，是否还没有定义最佳的表面处理来提高 YSZ 的生物活性。纹理化是一种很有前途的方法，但图案化 YSZ 表面在细胞培养中的生物学作用尚待确定。因此，研究了与凹槽纹理化的 YSZ 表面接触的成骨细胞和成纤维细胞的细胞行为。YSZ 圆盘通过常规铣削和 Nd:YAG 激光进行凹槽纹理处理。包括对照在内的所有样品都经过喷砂和酸蚀处理。人成骨细胞和成纤维细胞在圆盘上培养 14 天。观察形态和细胞粘附。细胞活力、白细胞介素-1β、骨桥蛋白、I 型胶原蛋白的产生、碱性磷酸酶活性、和白细胞介素8进行了测量。与激光纹理化相比，通过常规研磨的 YSZ 纹理化提高了成骨细胞的活力和分化。成纤维细胞的行为似乎不受纹理技术的影响。与目前使用的喷砂和酸蚀相比氧化锆牙种植体的金标准没有发现宏观纹理化的优越性。