Cellular uptake of nanoparticles plays a crucial role in cell-targeted biomedical applications. Despite abundant studies trying to understand the interaction between nanoparticles and cells, the influence of cell geometry traits such as cell spreading area and cell shape on the uptake of nanoparticles remains unclear. In this study, poly(vinyl alcohol) is micropatterned on polystyrene cell culture plates using ultraviolet photolithography to control the spreading area and shape of individual cells. The effects of these factors on the cellular uptake of poly(N-(2-hydroxypropyl)methacrylamide)-based micelles were investigated at a single-cell level. Human carcinoma MCF-7 and A549 cells as well as normal Hs-27 and MRC-5 fibroblasts were cultured on micropatterned surfaces. MCF-7 and A549 cells, both with larger sizes, had a higher total micelle uptake. However, the uptake of Hs-27 and MRC-5 cells decreased with increasing spreading area. In terms of cell shapes, MCF-7 and A549 cells with round shapes showed a higher micelle uptake, while those with a square shape had a lower cellular uptake. On the other hand, Hs-27 and MRC-5 cells showed opposite behaviors. The results indicate that the geometry of cells can influence the nanoparticle uptake and may shed light on the design of functional nanoparticles.

中文翻译：

---

调节微图案表面培养的细胞中基于聚 (N-(2-羟丙基) 甲基丙烯酰胺) 的胶束的摄取

纳米粒子的细胞摄取在细胞靶向生物医学应用中起着至关重要的作用。尽管有大量研究试图了解纳米粒子与细胞之间的相互作用，但细胞几何特征（如细胞扩散面积和细胞形状）对纳米粒子吸收的影响仍不清楚。在这项研究中，使用紫外光刻法在聚苯乙烯细胞培养板上对聚（乙烯醇）进行微图案化，以控制单个细胞的扩散区域和形状。在单细胞水平上研究了这些因素对基于聚 (N-(2-羟丙基) 甲基丙烯酰胺) 的胶束的细胞摄取的影响。在微图案表面上培养人癌 MCF-7 和 A549 细胞以及正常 Hs-27 和 MRC-5 成纤维细胞。MCF-7 和 A549 电池，尺寸都更大，具有较高的总胶束吸收。然而，Hs-27 和 MRC-5 细胞的摄取随着扩散面积的增加而减少。在细胞形状方面，圆形的MCF-7和A549细胞的胶束吸收率较高，而方形的细胞吸收率较低。另一方面，Hs-27 和 MRC-5 细胞表现出相反的行为。结果表明，细胞的几何形状可以影响纳米颗粒的吸收，并可能为功能性纳米颗粒的设计提供启示。