Micro/nano hierarchical substrates with different micropillar spacings were designed and prepared for capture of tumor cells. The cell capture efficiency of hierarchical substrates with low-density micropillar arrays was similar to that of nanostructured substrate. Increasing the density of micopillars could significantly improve the capture efficiency. The maximum capture efficiency was achieved on the hierarchical substrate with micropillar spacings of 15 μm, but further reducing the micropillar spacings did not increase the cell capture efficiency. It was also found that hierarchical substrates with appropriate spacing of micropillars appeared more favorable for cell attachment and spreading, and thus enhancing the cell-material interaction. These results suggested that optimizing the micropillar arrays, such as the spacing between adjacent micropillars, could give full play to the synergistic effect of hierarchical hybrid micro/nanostructures in the interaction with cells. This study may provide promising guidance to design and optimize micro/nano hierarchical structures of biointerfaces for biomedical application.

设计并制备了具有不同微柱间距的微/纳米分级基板，用于捕获肿瘤细胞。具有低密度微柱阵列的分层基板的细胞捕获效率与纳米结构基板的相似。增加微柱的密度可以显着提高捕获效率。在微柱间距为 15 μ的分层基板上实现了最大捕获效率m，但进一步减小微柱间距并没有提高细胞捕获效率。还发现具有适当微柱间距的分层基板似乎更有利于细胞附着和扩散，从而增强细胞与材料的相互作用。这些结果表明，优化微柱阵列，例如相邻微柱之间的间距，可以充分发挥分层混合微/纳米结构在与细胞相互作用中的协同作用。该研究可能为设计和优化用于生物医学应用的生物界面的微/纳米层次结构提供有希望的指导。