The present experiment was conducted to evaluate the mitigation strategy of the arsenic toxicity induced in Black Bengal kids through dietary supplementation of vitamin E or yeast culture (Saccharomyces cerevisiae) or combination of both. Thirty number of Black Bengal kids (6.15 ± 0.32 kg, 142.87 ± 9.28 days) were divided into equal five groups. Kids under control group (T₀) and other treatment groups (T₁, T₂, T₃ and T₄) were fed with similar type of basal diet containing concentrate mixture and sorghum sudan grass. Kids under T₁, T₂, T₃ and T₄ were fed with 50 ppm arsenic (As)/kg DM of feed in the form of sodium arsenite (NaAsO₂). Kids under T₂, T₃ and T₄ were supplemented with Vitamin E (250 IU/kg DM of feed), yeast culture (S. cerevisiae) (4 × 10⁹ CFU/kid/d) and combination of vitamin E and S. cerevisiae with same doses, respectively. The feeding experiment was continued for 20 weeks. Challenge feeding of arsenic (As) significantly (P < 0.001) reduced body weight gain in kids under T₁ (only As fed) than control T₀ (without As); however, weight gain in T₂, T₃ and T₄ was replenished due to supplementation of vitamin E or yeast culture (S. cerevisiae) or combination of both vitamin E and yeast culture. Similarly, ADG (g/d) was decreased (P < 0.01) in kids under T₁ than other treatments (T₀, T₂, T₃ and T₄). Induction of arsenic toxicity decreased (P < 0.001) intakes of DM (g/d/kg W^0.75) and CP (g/d/kg W^0.75) in kids. Feed conversion efficiency (growth as percentage of DMI) was lowest (P < 0.01) in arsenic fed kids (T₁). The values ranged from 8.38 (T₁) to 9.31 (T₀). However, comparable FCE was observed among T₀, T₂, T₃ and T₄. Supplementation of vitamin E or S. cerevisiae or combination of both have compensated the DMI, CPI, weight gain and ADG; consequently increased feed conversion efficiency in kids as compared to As fed kids (T₁). Arsenic load was significantly (P < 0.01) greater in faeces, hair and urine of As fed group (T₁); which was reduced (P < 0.01) by supplementation of vitamin E alone (T₂) or combination of vitamin E and S. cerevisiae (T₄). Supplementation of S. cerevisiae alone was not much effective in reducing the arsenic load in faeces or urine or hair in arsenic intoxicated animals. Challenge feeding of As for 140 days exhibited some clinical signs; like "raindrops on the dusty road” like symptom on skin, mouth lesions, rough skin, alopecia, visible pathological changes in some internal organs (like liver, lungs etc.) and diarrhoea. However, such symptoms were less pronounced in the kids supplemented with vitamin E or combination of vitamin E and yeast culture. Therefore, supplementation of vitamin E (@ 250 IU/kg DM of feed) either alone or along with S. cerevisiae (@ 4 × 10⁹ CFU/kid/d) was partially effective in ameliorating the voluntary feed intake, growth and arsenic load in faeces, urine and hair of Black Bengal kids induced with toxic dose of arsenic @ 50 mg/kg DM of feed as sodium arsenite.

本实验旨在评估通过膳食补充维生素 E 或酵母培养物（酿酒酵母）或两者的组合对黑孟加拉儿童诱导的砷毒性的缓解策略。三十个黑孟加拉儿童 (6.15 ± 0.32 kg, 142.87 ± 9.28 天) 被分成相等的五组。对照组（T ₀）和其他治疗组（T ₁、T ₂、T ₃和T ₄）的孩子用相似类型的含有浓缩混合物和高粱苏丹草的基础日粮喂养。T ₁、T ₂、T ₃和T ₄岁以下的儿童以亚砷酸钠 (NaAsO ₂ )形式喂食 50 ppm 砷 (As)/kg DM 饲料。T ₂、T ₃和T _{4 以下的}儿童补充维生素E（250 IU/kg DM 饲料）、酵母培养物（酿酒酵母）（4 × 10 ⁹ CFU/kid/d）以及维生素E 和S 的组合.分别具有相同剂量的酿酒酵母。喂养实验持续20周。挑战喂养砷 (As) 显着 (P < 0.001) 降低了 T ₁ (仅 As 喂养)下儿童的体重增加，而不是对照 T ₀ (无 As)；然而，T ₂、T ₃和 T ₄体重增加由于补充维生素 E 或酵母培养物（酿酒酵母）或维生素 E 和酵母培养物的组合而补充。类似地，与其他治疗（T ₀、T ₂、T ₃和T ₄）相比，T ₁下儿童的ADG（g/d）降低（P < 0.01 ）。砷中毒的诱导降低了 (P < 0.001)儿童DM (g/d/kg W ^0.75 ) 和 CP (g/d/kg W ^0.75 ) 的摄入量。在以砷喂养的孩子 (T ₁ ) 中，饲料转化效率（以 DMI 百分比表示的增长）最低 (P < 0.01 )。值的范围从 8.38 (T ₁ ) 到 9.31 (T ₀ )。然而，在 T 中观察到可比的 FCE₀、T ₂、T ₃和T ₄。补充维生素 E 或酿酒酵母或两者的组合补偿了 DMI、CPI、体重增加和 ADG；因此，与喂食儿童 (T ₁ )相比，增加了儿童的饲料转化效率。As 喂养组（T ₁）的粪便，头发和尿液中的砷负荷显着（P <0.01）更高；通过单独补充维生素 E (T ₂ ) 或维生素 E 和酿酒酵母的组合(T ₄ )降低 (P < 0.01 )。补充酿酒酵母单独使用对减少砷中毒动物粪便、尿液或毛发中的砷含量并没有多大效果。As 140 天的挑战喂养表现出一些临床症状；像“尘土飞扬的雨滴”等皮肤症状、口腔病变、皮肤粗糙、脱发、一些内脏（如肝、肺等）可见病理变化和腹泻。但这些症状在补充的孩子中不太明显与维生素 E 或维生素 E 和酵母培养物的组合。因此，单独或与酿酒酵母（@ 4 × 10 ⁹）一起补充维生素 E（@ 250 IU/kg DM 饲料） CFU/kid/d) 部分有效地改善了以亚砷酸钠形式存在的有毒剂量的砷@50 mg/kg DM 饲料诱导的黑孟加拉儿童的粪便、尿液和头发中的自愿采食量、生长和砷负荷。