Abstract

The effect of in ovo feeding of different levels of vitamins C and E on egg hatchability, immune response, growth and carcass traits of broiler chickens were investigated. A total of 672 fertilized eggs were assigned to one of eight experimental groups having three replicates with 28 eggs as follows: (1) negative control (not injected); (2) positive control (injected with 0.2 mL deionized water); (3) vitamin C at 1 mg; (4) vitamin C at 3 mg; (5) vitamin C at 6 mg; (6) vitamin E at 0.5 IU; (7) vitamin E at 0.75 IU; and (8) vitamin E at 1.0 IU. At the end of incubation, the number of chicks hatched, and their individual body weight were recorded. Among hatched birds, a total of 240 mixed chicks were randomly selected (30 subject per group equally shared in three pen floors). Chicks were vaccinated against Avian Influenza, Gumboro, Bronchitis, and Newcastle disease virus. Performance parameters were weekly evaluated until 42 days of age. At days 28 and 42, broiler serum and spleen and Bursa of Fabricius relative weight were assessed as well as on day 42 the carcass traits. From results, in ovo injection with 3 mg of vitamin C or 0.75 IU of vitamin E, increased significantly (p < .05) the embryos hatchability when compared to the negative control. However, body weight at hatch and growth performance parameters showed no differences among treatments. Similarly, in ovo concentrations of vitamins C or E showed no differences on carcass traits, immunity-related organs weight or immune response for anti-Newcastle disease hemagglutination-inhibition and total immunoglobulins against sheep red blood cells (SRBC) when compared to the control groups. Based on findings, it can be concluded that in ovo feeding vitamins E and C supported positively chicken embryos hatchability demonstrating the key-role as antioxidant agents; however, further studies are currently being evaluated.

摘要

卵内的作用研究饲喂不同水平维生素C和E对肉鸡种蛋孵化率、免疫反应、生长和胴体性状的影响。总共 672 个受精卵被分配到 8 个实验组中的一个，每组 3 个重复，每组 28 个卵子如下： (1) 阴性对照（未注射）；(2)阳性对照(注射0.2mL去离子水)；(3) 维生素 C 1 毫克；(4) 维生素 C 3 毫克；(5) 维生素 C 6 毫克；(6) 0.5 IU 的维生素 E；(7) 0.75 IU 的维生素 E；(8) 1.0 IU 的维生素 E。孵化结束时，记录孵出的小鸡数量和个体体重。在孵出的鸟类中，随机选择了总共 240 只混合雏鸡（每组 30 只受试者，平均分配在三个围栏层）。小鸡接种了禽流感、Gumboro、支气管炎、和新城疫病毒。每周评估性能参数直到 42 日龄。在第 28 天和第 42 天，评估了肉鸡血清和脾脏以及法氏囊的相对重量，并在第 42 天评估了胴体性状。从结果来看，与阴性对照相比，卵内注射 3 mg 维生素 C 或 0.75 IU 维生素 E 可显着提高 ( p  < .05) 胚胎孵化率。然而，孵化时的体重和生长性能参数在处理之间没有差异。同样，与对照组相比，卵内维生素 C 或 E 的浓度对屠体性状、免疫相关器官重量或抗新城疫血凝抑制的免疫反应和针对绵羊红细胞 (SRBC) 的总免疫球蛋白没有差异. 根据调查结果，可以得出结论，在卵饲喂维生素 E 和 C 对鸡胚孵化率有积极支持，证明其作为抗氧化剂的关键作用；然而，目前正在评估进一步的研究。