MicroRNAs (miRNAs) are small non-coding RNAs that play key roles in tumorigenesis as modulators of cell signaling pathways. miRNA expression has been found to be dysregulated in several human and canine tumors, but data are not yet available on canine meningioma. In this study, we analyzed the expression of 12 miRNAs (i.e. miR-335, miR-200a, miR-98, miR-96, miR-190a, miR-29c, miR-219-5p, miR-155, miR-146a, miR-145, miR-136, miR-451) by RT-qPCR in a series of 41 formalin-fixed, paraffin-embedded canine meningiomas, and normal arachnoid samples. We identified 8 dysregulated miRNAs that might be involved in canine meningioma pathogenesis. Five miRNAs (i.e. miR-96, miR-145, miR-335, miR-200a, miR-29c), were downregulated in tumor samples and 3 (i.e. miR-136, miR-155, miR-146a) were upregulated. Moreover, miR-200a was overexpressed in grade III compared to grade I and grade II meningiomas, suggesting that it might have a dual role in tumor initiation and progression. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses suggest that dysregulated miRNAs might influence cellular processes and pathways mainly involved in tumor cell migration, extracellular matrix interactions, cell proliferation, and inflammatory responses. The characterization of miRNA functions in canine meningiomas is needed to assess their potential clinical utility, also in view of the relevance of the dog as a potential spontaneous animal model of human disease.

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犬脑膜瘤中的 MicroRNA 失调：福尔马林固定石蜡包埋样品的 RT-qPCR 分析

微小 RNA (miRNA) 是小的非编码 RNA，在肿瘤发生中作为细胞信号通路的调节剂发挥关键作用。已发现 miRNA 表达在几种人类和犬类肿瘤中失调，但尚无关于犬类脑膜瘤的数据。在这项研究中，我们分析了 12 种 miRNA（即 miR-335、miR-200a、miR-98、miR-96、miR-190a、miR-29c、miR-219-5p、miR-155、miR-146a , miR-145, miR-136, miR-451) 通过 RT-qPCR 在一系列 41 个福尔马林固定、石蜡包埋的犬脑膜瘤和正常蛛网膜样本中进行。我们确定了 8 种失调的 miRNA，它们可能与犬脑膜瘤发病机制有关。5 种 miRNA（即 miR-96、miR-145、miR-335、miR-200a、miR-29c）在肿瘤样品中下调，3 种（即 miR-136、miR-155、miR-146a）上调。而且，与 I 级和 II 级脑膜瘤相比，miR-200a 在 III 级过表达，表明它可能在肿瘤发生和进展中具有双重作用。基因本体论和京都基因百科全书和基因组通路富集分析表明，失调的 miRNA 可能影响主要涉及肿瘤细胞迁移、细胞外基质相互作用、细胞增殖和炎症反应的细胞过程和通路。需要对犬脑膜瘤中的 miRNA 功能进行表征，以评估其潜在的临床效用，同时考虑到狗作为人类疾病潜在自发动物模型的相关性。基因本体论和京都基因百科全书和基因组通路富集分析表明，失调的 miRNA 可能影响主要涉及肿瘤细胞迁移、细胞外基质相互作用、细胞增殖和炎症反应的细胞过程和通路。需要对犬脑膜瘤中的 miRNA 功能进行表征，以评估其潜在的临床效用，同时考虑到狗作为人类疾病潜在自发动物模型的相关性。基因本体论和京都基因百科全书和基因组通路富集分析表明，失调的 miRNA 可能影响主要涉及肿瘤细胞迁移、细胞外基质相互作用、细胞增殖和炎症反应的细胞过程和通路。需要对犬脑膜瘤中的 miRNA 功能进行表征，以评估其潜在的临床效用，同时考虑到狗作为人类疾病潜在自发动物模型的相关性。