Black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), larvae are suspected to inhibit house flies, Musca domestica (L.) (Diptera: Muscidae), seeking oviposition sites. However, the mechanisms regulating these interactions are not well known. In this study, a Y-tube olfactometer was used to determine whether black soldier fly eggs, larvae, puparia (without pupae inside), adult carcasses, or resulting frass of black soldier fly decrease attractiveness of adult house flies to an oviposition substrate (i.e., sugar and wheat bran diet, used as the control). Secondly, two symbiotic bacteria associated were isolated, identified, and tested for house fly attractiveness with a Y-tube olfactometer. Thirdly, volatile organic compounds produced by swine feed before and after black soldier fly, or house fly larval digestion were analyzed with gas chromatography–mass spectrometry (GC–MS). Results indicate feed mixed with black soldier fly larval frass, and symbiotic bacteria, Paenalcaligenes sp. associated with black soldier fly, were less attractive to house flies than the control. However, another symbiotic bacterium Lysinibacillus sp. associated with black soldier fly pupae was attractive. In total, 52 volatile organic compounds were identified from treatments previously described. Quantitative differences in volatile profiles across treatments potentially explain the mechanism regulating house fly substrate choices in the presence, or absence, of black soldier fly larvae.

中文翻译：

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成年家蝇（双翅目：蝇科）对黑兵蝇（双翅目：地层蝇科）的反应相关底物和潜在挥发性有机化合物鉴定

黑水虻、 Hermetia illucens (L.) (双翅目：Stratiomyidae)、幼虫被怀疑抑制家蝇、Musca domestica (L.) (双翅目：Muscidae)，寻找产卵场所。然而，调节这些相互作用的机制尚不清楚。在这项研究中，使用 Y 型管嗅觉计来确定黑水虻的卵、幼虫、蛹（内部没有蛹）、成虫尸体或由此产生的黑水虻碎屑是否会降低成年家蝇对产卵基质的吸引力（即，糖和麦麸饮食，用作对照）。其次，分离、鉴定了两种相关的共生细菌，并用 Y 型管嗅觉仪测试了家蝇的吸引力。三、黑水虻前后猪饲料产生的挥发性有机物，用气相色谱 - 质谱（GC-MS）分析或家蝇幼虫消化。结果表明，饲料中混有黑水虻幼虫和共生细菌 Paenalcaligenes sp。与黑水虻有关，对家蝇的吸引力不如对照。然而，另一种共生细菌 Lysinibacillus sp。与黑水虻蛹有关的东西很有吸引力。从先前描述的处理中总共鉴定出 52 种挥发性有机化合物。不同处理中挥发性成分的定量差异可能解释了在存在或不存在黑水虻幼虫的情况下调节家蝇基质选择的机制。与黑水虻有关，对家蝇的吸引力不如对照。然而，另一种共生细菌 Lysinibacillus sp。与黑水虻蛹有关的东西很有吸引力。从先前描述的处理中总共鉴定出 52 种挥发性有机化合物。不同处理中挥发性成分的定量差异可能解释了在存在或不存在黑水虻幼虫的情况下调节家蝇基质选择的机制。与黑水虻有关，对家蝇的吸引力不如对照。然而，另一种共生细菌 Lysinibacillus sp。与黑水虻蛹有关的东西很有吸引力。从先前描述的处理中总共鉴定出 52 种挥发性有机化合物。不同处理中挥发性成分的定量差异可能解释了在存在或不存在黑水虻幼虫的情况下调节家蝇基质选择的机制。