Over the past few years, research tools have been developed to monitor the multistep protein aggregation process in live cells, a process that has been associated with a growing number of human diseases. Herein, we describe recent advances in methods that can either survey the distribution of aggregation at the level of the cellular proteome using mass spectroscopy or discern the multistep aggregation process of specific proteins of interest via fluorescence signals. Future development and application of such technologies are expected to provide insights on mechanisms, diagnosis, and treatment of diseases rooted in protein aggregation.

中文翻译：

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活细胞中蛋白质错误折叠和聚集的直接可视化和分析。

在过去的几年里，已经开发出研究工具来监测活细胞中的多步蛋白质聚集过程，这一过程与越来越多的人类疾病有关。在这里，我们描述了方法的最新进展，这些方法既可以使用质谱法调查细胞蛋白质组水平的聚集分布，也可以通过荧光信号辨别感兴趣的特定蛋白质的多步聚集过程。此类技术的未来发展和应用有望为根源于蛋白质聚集的疾病的机制、诊断和治疗提供见解。