Abstract

Although wide variety of molecular precursors have been used for carbonization-based synthesis of fluorescent carbon nanoparticle, biomolecule-derived carbon nanoparticle with tunable fluorescence is difficult to synthesize. Here, we report a generalized approach for the preparation of fluorescent carbon nanoparticle from various biomolecules, such as lactose, ascorbic acid, tyrosine and sucrose. The method involves controlled carbonization of molecular precursor in ethylene glycol at 190°C in the presence of Na₃PO₄. The presented synthetic method can produce 20–30 mg of nanoparticles in one batch with fluorescence quantum yield in the range of 1–10% and nanoparticles can be conjugated with primary amine-terminated chemical/biochemical by simple incubation. These fluorescent carbon nanoparticles can be transformed into different nanobioconjugates for various biomedical applications.

中文翻译：

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生物分子衍生和功能化的荧光碳纳米粒子的广义合成

摘要

尽管各种各样的分子前体已被用于基于碳化的荧光碳纳米颗粒的合成，但难以合成具有可调荧光的生物分子衍生的碳纳米颗粒。在这里，我们报告了一种从各种生物分子（如乳糖、抗坏血酸、酪氨酸和蔗糖）制备荧光碳纳米颗粒的通用方法。该方法包括在 190°C 和 Na ₃ PO ₄存在下，在乙二醇中控制分子前体碳化. 所提出的合成方法可以在一批中生产 20-30 毫克纳米颗粒，荧光量子产率在 1-10% 的范围内，纳米颗粒可以通过简单的孵育与伯胺封端的化学/生物化学结合。这些荧光碳纳米粒子可以转化为不同的纳米生物共轭物，用于各种生物医学应用。