The major challenge in utilizing pesticides lies in identifying the precise application that would improve the efficiency of these pesticides and decline their environmental and health hazards at the same time. Such application requires the development of specific formulations that enable controlled, stimuli-responsive release of the pesticides. Gelatin is a relatively cheap material characterized by temperature-sensitivity and abundant amino acid groups, which makes it suitable for the storage and controlled release of pesticides. In this study, gelatin microspheres were prepared by emulsion and cross-linking, then they were loaded with 2,4-dichlorophenoxyacetic acid sodium (2,4-D Na) as a model herbicide. To achieve temperature-tunable release of 2,4-D Na from the microspheres, NH₄HCO₃ was added to the formulations at different concentrations. The prepared formulations were characterized by SEM, FTIR, and size distribution analyzes, and their drug loading capacities were determined. Based on bioassay experiments, the 2,4-D Na-NH₄HCO₃-loaded gelatin microspheres can effectively control the spread of dicotyledonous weeds. Therefore, the strategy proposed herein can be used to develop novel, effective herbicide formulations.

使用杀虫剂的主要挑战在于确定可以提高这些杀虫剂效率并同时降低其对环境和健康危害的精确应用。此类应用需要开发能够控制杀虫剂的刺激响应释放的特定配方。明胶是一种相对廉价的材料，具有温度敏感性和丰富的氨基酸基团等特点，适用于农药的储存和控释。本研究通过乳化和交联制备明胶微球，然后负载2,4-二氯苯氧基乙酸钠（2,4-D Na）作为除草剂模型。为了实现从微球中温度可调地释放 2,4-D Na，NH ₄ HCO ₃以不同浓度添加到配方中。通过 SEM、FTIR 和粒度分布分析对制备的制剂进行表征，并确定其载药能力。基于生物测定实验，负载2,4-D Na-NH ₄ HCO _{3 的}明胶微球可以有效控制双子叶杂草的蔓延。因此，本文提出的策略可用于开发新的、有效的除草剂制剂。