DNA origami can be applied as a “ruler” for nanoscale calibration or super-resolution fluorescence microscopy with an ideal structure for defining fluorophore arrangement, allowing the distance between fluorophores to be precisely controlled at the nanometer scale. DNA origami can also be used as a nanotag with arbitrary programmable shapes for topological identification. In this study, we formed a hexagonal origami structure embedded with three different fluorescent dyes on the surface. The distance between each fluorescent block was ~120 nm, which is below the diffraction limit of light, allowing for its application as a nano-ruler for super-resolution fluorescence microscopy. The outside edge of the hexagonal structure was redesigned to form three different substructures as topological labels. Atomic and scanning force microscopy demonstrated the consistent nanoscale distance between morphological and fluorescent labels. Therefore, this fluorophore-embedded hexagonal origami platform can be used as a dual nanoruler for optical and topological calibration.

中文翻译：

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六角形 DNA 折纸纳米标签的光学和拓扑表征


DNA折纸可以作为纳米级校准或超分辨率荧光显微镜的“尺子”，具有定义荧光团排列的理想结构，允许在纳米尺度上精确控制荧光团之间的距离。 DNA折纸还可以用作具有任意可编程形状的纳米标签，用于拓扑识别。在这项研究中，我们形成了一种六边形折纸结构，表面嵌入了三种不同的荧光染料。每个荧光块之间的距离约为 120 nm，低于光的衍射极限，使其可以用作超分辨率荧光显微镜的纳米尺。六边形结构的外缘经过重新设计，形成三种不同的子结构作为拓扑标签。原子和扫描力显微镜证明了形态和荧光标记之间一致的纳米级距离。因此，这种嵌入荧光团的六边形折纸平台可以用作光学和拓扑校准的双纳米尺。