Introduction and objectives: With increasing pet allergies among pediatric patients, the need for precise environmental care is increasing. We investigated the clinical, immunological, and environmental characteristics of pediatric patients sensitized to a dog to evaluate the cross-antigenicity of canine lipocalin Can f 1 with feline lipocalin Fel d 1 and Syrian hamster extract.Materials and methods: The protein fractions of the processed and commercial Syrian hamster extracts were compared using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). An enzyme-linked immunosorbent assay (ELISA) inhibition test was performed on Can f 1, Fel d 1, and processed Syrian hamster extract, and the antigen-specific immunoglobulin E (IgE)-binding capacity for each antigen was analyzed using serum samples from patients.Results: Twelve of 19 patients with a median age of 40.5 months were symptomatic when exposed to dogs. Eleven (91.7%) patients showed a positive IgE response to Can f 1. Two patients were positive for Fel d 1-specific IgE antibody, and one was positive for hamster-specific IgE antibody. SDS-PAGE confirmed the presence of different patterns of protein bands between the commercial and processed hamster extracts. There was no cross-antigenicity among Can f 1, Fel d 1, and processed Syrian hamster extract.

中文翻译：

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Can f 1 与叙利亚仓鼠和儿童 Fel d 1 的交叉反应。

介绍和目标：随着儿科患者对宠物过敏的增加，对精确环境护理的需求也在增加。我们调查了对狗敏感的儿科患者的临床、免疫学和环境特征，以评估犬 lipocalin Can f 1 与猫 lipocalin Fel d 1 和叙利亚仓鼠提取物的交叉抗原性。材料和方法：加工过的蛋白质组分使用十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳 (SDS-PAGE) 比较了叙利亚仓鼠和商业叙利亚仓鼠提取物。对 Can f 1、Fel d 1 和加工过的叙利亚仓鼠提取物进行酶联免疫吸附试验 (ELISA) 抑制试验，并使用来自患者。结果：19 名中位年龄为 40.5 个月的患者中有 12 名在与狗接触时出现症状。11 名 (91.7%) 患者对 Can f 1 显示出阳性 IgE 反应。2 名患者 Fel d 1 特异性 IgE 抗体阳性，1 名患者特异性 IgE 抗体阳性。SDS-PAGE 证实了商业和加工仓鼠提取物之间存在不同模式的蛋白质条带。Can f 1、Fel d 1 和加工过的叙利亚仓鼠提取物之间没有交叉抗原性。SDS-PAGE 证实了商业和加工仓鼠提取物之间存在不同模式的蛋白质条带。Can f 1、Fel d 1 和加工过的叙利亚仓鼠提取物之间没有交叉抗原性。SDS-PAGE 证实了商业和加工仓鼠提取物之间存在不同模式的蛋白质条带。Can f 1、Fel d 1 和加工过的叙利亚仓鼠提取物之间没有交叉抗原性。