Background: Although whole-body cooling has been reported to improve the ischemic/reperfusion injury in hemorrhagic shock (HS) resuscitation, it is limited by its adverse reactions following therapeutic hypothermia. HS affects the experimental and clinical bowel disorders via activation of the brain-gut axis. It is unknown whether selective brain cooling achieves beneficial effects in HS resuscitation via preserving the integrity of the brain-gut axis./nMethods: Male Sprague-Dawley rats were bled to hypovolemic HS and resuscitated with blood transfusion followed by retrograde jugular vein flush (RJVF) with 4 °C or 36 °C normal saline. The mean arterial blood pressure, cerebral blood flow, and brain and core temperature were measured. The integrity of intestinal tight junction proteins and permeability, blood pro-inflammatory cytokines, and multiple organs damage score were determined./nResults: Following blood transfusion resuscitation, HS rats displayed gut barrier disruption, increased blood levels of pro-inflammatory cytokines, and peripheral vital organ injuries. Intrajugular-based infusion cooled the brain robustly with a minimal effect on body temperature. This brain cooling significantly reduced the HS resuscitation-induced gut disruption, systemic inflammation, and peripheral vital organ injuries in rats./nConclusion: Resuscitation with selective brain cooling achieves peripheral vital organs protection in hemorrhagic shock resuscitation via preserving the integrity of the brain-gut axis.

中文翻译：

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选择性脑冷却通过保持脑肠轴的完整性在失血性休克复苏中实现外周器官保护

背景：虽然有报道称全身冷却可以改善失血性休克 (HS) 复苏中的缺血/再灌注损伤，但它受到治疗性低温后的不良反应的限制。HS 通过激活脑肠轴影响实验和临床肠道疾病。尚不清楚选择性脑冷却是否通过保持脑 - 肠轴的完整性在 HS 复苏中实现有益效果。/n方法：雄性 Sprague-Dawley 大鼠被放血至低血容量 HS 并通过输血复苏，然后用 4°C 或 36°C 生理盐水逆行颈静脉冲洗 (RJVF)。测量平均动脉血压、脑血流量以及大脑和核心温度。确定了肠道紧密连接蛋白的完整性和通透性、血液促炎细胞因子和多器官损伤评分。/n结果：输血复苏后，HS 大鼠表现出肠道屏障破坏、促炎细胞因子血液水平升高和外周重要器官损伤。基于颈内静脉的输注有力地冷却大脑，对体温的影响最小。这种脑冷却显着减少了大鼠 HS 复苏引起的肠道破坏、全身炎症和外周重要器官损伤。/n结论：选择性脑冷却复苏通过保持脑的完整性来实现失血性休克复苏中的外周重要器官保护——肠轴。