Antonie van Leeuwenhoek ( IF 1.8 ) Pub Date : 2021-06-25 , DOI: 10.1007/s10482-021-01607-6 Ljubov Dzanaeva 1 , Barbara Kruk 2 , Justyna Ruchala 2 , Andriy Sibirny 1, 2 , Kostyantyn Dmytruk 1
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Abstract
Lignocellulosic biomass is an attractive sustainable platform for fuel ethanol production. Xylose is a second after glucose most abounded sugar in lignocellulosic hydrolysates. Effective conversion of xylose to ethanol is one of key prerequisite for the development of an efficient conversion of biomass to ethanol. Engineered Saccharomyces cerevisiae strains are able to xylose fermentation. However, the yield and productivities of xylose fermentation remains lower in comparison with glucose fermentation. In this work, we studied impact of transcription factors Znf1, Sip4, Adr1, Tup1, and Hap4 on xylose catabolism. We have isolated znf1Δ, adr1Δ, tup1Δ and hap4Δ mutants, and strains overexpressing SIP4, ADR1 and HAP4 genes on the background of xylose-fermenting strain of S. cerevisiae aiming to explore involvement of these transcription factors in regulation of xylose growth and fermentation. It was shown that hap4Δ reveal 1.8-fold increase of ethanol production from xylose as compared to that of parental strain. The hap4Δ mutant accumulates 10.38 g l−1 of ethanol with an overall ethanol yield reaching 0.41 g g−1 of consumed xylose. While the other constructed strains revealed a decrease in ethanol production from this pentose.
Graphical Abstract
中文翻译：
转录因子 Znf1、Sip4、Adr1、Tup1 和 Hap4 对工程酵母酿酒酵母中木糖酒精发酵的影响
摘要
木质纤维素生物质是燃料乙醇生产的一个有吸引力的可持续平台。木糖仅次于葡萄糖，在木质纤维素水解物中含量最高。木糖向乙醇的有效转化是发展生物质向乙醇的有效转化的关键先决条件之一。工程酿酒酵母菌株能够进行木糖发酵。然而，与葡萄糖发酵相比，木糖发酵的产量和生产率仍然较低。在这项工作中，我们研究了转录因子 Znf1、Sip4、Adr1、Tup1 和 Hap4 对木糖分解代谢的影响。我们已经分离出znf1Δ、adr1Δ、tup1Δ和hap4Δ突变体，以及过表达SIP4、ADR1 的菌株和HAP4基因在酿酒酵母木糖发酵菌株的背景下，旨在探索这些转录因子参与木糖生长和发酵的调节。结果表明，与亲本菌株相比，hap4Δ显示来自木糖的乙醇产量增加了 1.8 倍。所述hap4Δ突变体累积10.38克升-1的乙醇具有总体乙醇产量达到0.41克克-1消耗木糖。而其他构建的菌株显示这种戊糖的乙醇产量下降。
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