In this study, the silk fibroin/nano-hydroxyapatite/hyaluronic acid (SF/nHAp/HA) composite scaffolds with different HA contents were developed by blending, cross-linking and freeze-drying, and their physicochemical properties and cell biocompatibility in vitro were subsequently studied. It was observed that the molecular conformation of the composite scaffolds was mainly composed of silk I and a small amount of the β-sheets structure. On enhancing the HA content, the pore size of the scaffold decreased, while the porosity, water absorption, swelling ratio and mechanical properties were observed to increase. In particular, the SF/nHAp/HA scaffold with a 5.0 wt% ratio exhibited the highest water absorption and mechanical properties among the developed materials. In addition, the in vitro cytocompatibility analysis showed that the bone marrow mesenchymal stem cells exhibited excellent cell proliferation and osteogenic differentiation ability on the SF/nHAp/5.0 wt%HA scaffolds, as compared with the other scaffolds. It can be concluded that the developed composite scaffolds represent a promising class of materials for the bone tissue repair and regeneration.

本研究通过共混、交联、冷冻干燥等方法制备了不同HA含量的丝素蛋白/纳米羟基磷灰石/透明质酸（SF/nHAp/HA）复合支架，并对其理化性质和体外细胞生物相容性进行了研究。后来学习了。观察到复合支架的分子构象主要由丝I和少量的β-折叠结构组成。随着HA含量的增加，支架的孔径减小，而孔隙率、吸水率、溶胀率和机械性能增加。特别是，比例为 5.0 wt% 的 SF/nHAp/HA 支架在所开发的材料中表现出最高的吸水率和机械性能。此外，体外细胞相容性分析表明，与其他支架相比，骨髓间充质干细胞在SF/nHAp/5.0 wt%HA支架上表现出优异的细胞增殖和成骨分化能力。可以得出结论，所开发的复合支架代表了一类有前途的骨组织修复和再生材料。