Infantile pneumonia (IP) is an acute lower respiratory infection and brings a heavy burden to children health. Circular RNAs (circRNAs) participate in the regulation of pneumonia process. In this research, the effects of circ_0038467 in regulating lipopolysaccharide (LPS)-induced cell injury and underlying mechanism were revealed. Results showed that circ_0038467 expression and TLR4 protein level were upregulated, while miR-195-5p expression was downregulated in LPS-induced MRC-5 cells. Circ_0038467 silencing restored LPS-mediated inhibition on cell proliferation and promotion on apoptosis and inflammatory response. Additionally, circ_0038467 acted as a sponge of miR-195-5p, which was further revealed to target TLR4. MiR-195-5p inhibitor reversed circ_0038467 silencing-mediated influences under LPS treatment. Furthermore, LPS-activated NF-κB pathway was partly blocked by circ_0038467 silencing, which was restrained by TLR4 overexpression. Circ_0038467 silencing protected MRC-5 cells from LPS-induced injury by miR-195-5p/TLR4 axis through NF-κB pathway, providing a theoretical basis for circRNA-directed IP therapy.

中文翻译：

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Circ_0038467 在 MRC-5 细胞中通过 NF-κB 通路通过 miR-195-5p/TLR4 轴调节脂多糖介导的细胞增殖、凋亡和炎症反应。

婴儿肺炎（IP）是一种急性下呼吸道感染，给儿童健康带来沉重负担。环状RNA（circRNA）参与了肺炎过程的调控。本研究揭示了circ_0038467在调节脂多糖（LPS）诱导的细胞损伤中的作用及其潜在机制。结果表明，在LPS诱导的MRC-5细胞中，circ_0038467表达和TLR4蛋白水平上调，而miR-195-5p表达下调。Circ_0038467 沉默恢复了 LPS 介导的细胞增殖抑制和细胞凋亡和炎症反应的促进。此外，circ_0038467 充当 miR-195-5p 的海绵，进一步揭示其靶向 TLR4。MiR-195-5p 抑制剂逆转了 LPS 治疗下 circ_0038467 沉默介导的影响。此外，LPS 激活的 NF-κB 通路被 circ_0038467 沉默部分阻断，这被 TLR4 过表达抑制。Circ_0038467 沉默保护MRC-5 细胞免受miR-195-5p/TLR4 轴通过NF-κB 通路引起的LPS 损伤，为circRNA 指导的IP 治疗提供了理论基础。