The knowledge of the diversity and genetic structure of pest insects under management contributes to the improvement of control strategies. An experiment was run to investigate whether the addition of the fungus Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) (BB) and compost to soil affects the presence and genetic diversity of adults and larvae of Phyllophaga obsoleta Blanch (Coleoptera: Melolonthinae) larvae in maize crops. We collected adults in and used mating pairs under four treatments (BB, compost, soil, blank). Genetic diversity and structure were determined through five allo/iso-enzymatic loci. Beauveria bassiana affected the presence and mortality of P. obsoleta in the laboratory but not under field conditions. The genetic diversity of P. obsoleta ranged from moderate to high (Ho = 0.26–0.31), with a low genetic differentiation among localities or treatments (Phi < 0.05), indicating high levels of gene flow. Our results showed a weak effect of B. bassiana on P. obsoleta in the field. Still, our laboratory observations suggest that the fungus may be a suitable alternative for biological control.

中文翻译：

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白僵菌和堆肥在玉米作物上的应用如何影响淘汰金叶菜（鞘翅目：Melolonthinae）的生存和遗传多样性？

了解所管理害虫的多样性和遗传结构有助于改进控制策略。进行了一项实验以研究在土壤中添加真菌白僵菌 (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) (BB) 和堆肥是否会影响 Phylophaga obsoleta Blanch (Coleoptera: Melolonthinae) 幼虫的成虫和幼虫的存在和遗传多样性在玉米作物中。我们在四种处理（BB、堆肥、土壤、空白）下收集成年人并使用交配对。通过五个同种异体/异酶基因座确定遗传多样性和结构。Beauveria bassiana 在实验室中影响 P. obsoleta 的存在和死亡率，但在现场条件下没有影响。P. obsoleta 的遗传多样性从中等到高（Ho = 0.26-0.31），区域或处理之间的遗传分化低（Phi < 0.05），表明高水平的基因流动。我们的结果表明 B. bassiana 对田间 P. obsoleta 的影响较弱。尽管如此，我们的实验室观察表明，这种真菌可能是生物防治的合适替代品。