Combining two or more nanoparticles is a promising approach. Previously we have reported synthesis of nanoparticles Dysprosium (Dy) substituted with manganese (Mn) zinc (Zn) by using ultrasonication method. The five different nanoparticles (NPs) Mn0.5Zn0.5DyxFe2-xO4 (x ≤ 0.1) have been structurally and morphologically characterized but there is no report on the biological application of these NPs. In the present study, we have examined the anti-cancer, anti-bacterial, and anti-fungal activities of Mn0.5Zn0.5DyxFe2-xO4 (x ≤ 0.1) NPs. Human colorectal carcinoma cells (HCT-116) were tested with different concentrations of NPs by using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. In addition, the impact of NPs was also examined on normal cells such as human embryonic kidney cells, HEK-293. After 48 h of treatment, Mn0.5Zn0.5DyxFe2-xO4 NPs (x = 0.02, 0.04 and 0.06) showed no inhibitory action on cancer cell's growth and proliferation, whereas Mn0.5Zn0.5DyxFe2-xO4 NPs (x = 0.08 and 0.1) showed profound inhibitory action on cancer cell's growth and proliferation. However, the treatment of Mn0.5Zn0.5DyxFe2-xO4 NPs on the normal cells (HEK-293) did not show cytotoxic or inhibitory action on HEK-293 cells. The treatment of Mn0.5Zn0.5DyxFe2-xO4 NPs (x ≤ 0.1) also inhibited both the bacteria (Escherichia coli ATCC35218 and Staphylococcus aureus) with lowest MIC and MBC values of 4 and 8 mg/mL and fungus (Candida albicans) with MIC and MFC values of 4 and 8 mg/mL on treatment with x = 0.08 and 0. 1.

中文翻译：

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Mn0.5Zn0.5DyxFe2-xO4 (x ≤ 0.1) 纳米粒子的抗菌和抗癌活性。


结合两种或多种纳米粒子是一种有前途的方法。此前我们报道了采用超声法合成锰（Mn）锌（Zn）取代镝（Dy）纳米粒子。五种不同的纳米颗粒（NP）Mn0.5Zn0.5DyxFe2-xO4（x ≤ 0.1）已被结构和形态表征，但没有关于这些纳米颗粒的生物应用的报道。在本研究中，我们研究了Mn0.5Zn0.5DyxFe2-xO4 (x ≤ 0.1) NPs的抗癌、抗菌和抗真菌活性。使用 MTT [3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑]测定法对人结直肠癌细胞 (HCT-116) 进行不同浓度的 NP 测试。此外，还研究了纳米颗粒对正常细胞（例如人胚胎肾细胞 HEK-293）的影响。处理48小时后，Mn0.5Zn0.5DyxFe2-xO4 NPs（x = 0.02、0.04和0.06）对癌细胞的生长和增殖没有表现出抑制作用，而Mn0.5Zn0.5DyxFe2-xO4 NPs（x = 0.08和0.1）对癌细胞的生长和增殖显示出深刻的抑制作用。然而，Mn0.5Zn0.5DyxFe2-xO4 NPs对正常细胞(HEK-293)的处理并未显示出对HEK-293细胞的细胞毒性或抑制作用。 Mn0.5Zn0.5DyxFe2-xO4 NPs (x ≤ 0.1) 的处理还抑制细菌（大肠杆菌 ATCC35218 和金黄色葡萄球菌）（最低 MIC 和 MBC 值分别为 4 和 8 mg/mL）和真菌（白色念珠菌）（最低 MIC） x = 0.08 和 0. 1 处理时的 MFC 值为 4 和 8 mg/mL。