An efficient method for simultaneous extraction, purification and determination of six lignans in Schisandra chinensis Baill was developed by employing matrix solid-phase dispersion (MSPD) extraction followed by HPLC-UV determination analysis. Several sorbent and desorption solvent that affected the extraction yield of lignans were investigated; neutral alumina and absolute ethanol were selected as the best dispersing material and desorption agent, respectively. Other extraction conditions for MSPD were optimized as follows: 1:2 of S. chinensis raw material to neutral aluminum oxide mass ratio, 1:30 (g/mL) of sample to absolute ethanol, 2.5 h of desorption time and 50°C of desorption temperature. Under the above conditions, the total extraction yield for six lignans have reached (16.99 ± 0.33) x 103 mg/kg with a higher content of 6.88 ± 0.25% in the extracts. Comparative studies were explored by conducting other six extraction approaches including Soxhlet extraction, heat reflux extraction, smashing tissue extraction, microwave-assisted extraction, ultrasonic-assisted extraction and ultrasonic-microwave synergistic extraction. Results showed MSPD technique not only improved the extraction yield, but also improved the purity of lignans, it can be generalized to more extraction of natural compounds. In addition, the validated HPLC-UV method had been successfully applied to analysis of lignans from 10 real S. chinensis samples.

中文翻译：

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基质固相分散与高效液相色谱-紫外联用同时提取、纯化和测定五味子果实中六种木脂素

采用基质固相分散法（MSPD）萃取，HPLC-UV测定分析，建立了同时提取、纯化和测定五味子中六种木脂素的有效方法。研究了影响木脂素提取率的几种吸附剂和解吸溶剂；分别选用中性氧化铝和无水乙醇作为最佳分散材料和解吸剂。MSPD 的其他萃取条件优化如下： 1:2 的 S. chinensis 原料与中性氧化铝的质量比，1:30 (g/mL) 的样品与无水乙醇，2.5 h 的解吸时间和 50°C解吸温度。在上述条件下，六种木脂素的总提取量达到（16.99±0.33）×103 mg/kg，含量较高为6.88±0。提取物中的 25%。对索氏提取法、热回流提取法、粉碎组织提取法、微波辅助提取法、超声辅助提取法和超声-微波协同提取法等六种提取方法进行了比较研究。结果表明，MSPD技术不仅提高了提取率，而且提高了木脂素的纯度，可推广到更多天然化合物的提取。此外，经验证的 HPLC-UV 方法已成功应用于 10 份真实 S. chinensis 样品的木脂素分析。超声辅助提取和超声-微波协同提取。结果表明，MSPD技术不仅提高了提取率，而且提高了木脂素的纯度，可推广到更多天然化合物的提取。此外，经验证的 HPLC-UV 方法已成功应用于 10 份真实 S. chinensis 样品的木脂素分析。超声辅助提取和超声-微波协同提取。结果表明，MSPD技术不仅提高了提取率，而且提高了木脂素的纯度，可推广到更多天然化合物的提取。此外，经验证的 HPLC-UV 方法已成功应用于 10 份真实 S. chinensis 样品的木脂素分析。