Objective

To study the function of the RNA-binding protein Hfq in Bacillus subtilis cellulose decomposition.

Results

In the medium with sodium carboxymethylcellulose (Na-CMC) as the sole carbon source, the knockout of Hfq resulted in a 38.0% ± 2.1% and 76.6% ± 7.1% decrease in cellulose hydrolysis ability and cellulase activity, respectively. The results of real-time quantitative PCR revealed that several cellulase genes (eglS, bglA, and bglC) were significantly downregulated in the Hfq knockout strain. The isogenic Δhfq complemented strain recovered the cellulose hydrolysis ability, cellulase activity, and expression level of cellulase genes. In addition, the survival of Hfq mutant in stationary phase was significantly affected.

Conclusion

RNA-binding protein Hfq is involved in the regulation of cellulose hydrolysis ability, cellulase activity, cellulase gene expression, and stationary phase survival.

中文翻译：

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RNA结合蛋白Hfq在整个影响纤维素酶基因表达的纤维素分解中起着至关重要的作用

客观的

研究RNA结合蛋白Hfq在枯草芽孢杆菌纤维素分解中的作用。

结果

在以羧甲基纤维素钠 (Na-CMC) 作为唯一碳源的培养基中，敲除 Hfq 导致纤维素水解能力和纤维素酶活性分别下降 38.0% ± 2.1% 和 76.6% ± 7.1%。实时定量 PCR 的结果表明，几个纤维素酶基因（eglS、bglA和bglC）在 Hfq 敲除菌株中显着下调。等基因Δhfq互补菌株恢复了纤维素水解能力、纤维素酶活性和纤维素酶基因的表达水平。此外，Hfq突变体在静止期的存活受到显着影响。

结论

RNA 结合蛋白 Hfq 参与调节纤维素水解能力、纤维素酶活性、纤维素酶基因表达和静止期存活。