Since the removal of contaminations in microalgal cultures is extremely laborious and time-consuming, we developed a rapid workflow to obtain axenicity by a combination of fluorescence-activated cell sorting (FACS) and plate spreading. During method development, several cyanobacteria and green algae strains were successfully made axenic. At the end, method transferability to another FACS device was demonstrated. Our workflow offers great time-savings with less hands-on laboratory work compared to conventional isolation techniques.

中文翻译：

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使微藻培养物再次无菌 - 利用荧光激活细胞分选的快速而简单的工作流程。

由于去除微藻培养物中的污染物极其费力和耗时，我们开发了一种快速工作流程，通过结合荧光激活细胞分选 (FACS) 和平板铺展来获得无菌性。在方法开发过程中，几种蓝藻和绿藻菌株被成功制成无菌。最后，证明了方法可转移到另一个 FACS 设备。与传统的分离技术相比，我们的工作流程可以节省大量时间，减少实验室动手操作。