Severe radiation-induced skin injury is a complication of tumor radiotherapy and nuclear accidents. Cell therapy is a potential treatment for radiation-induced skin injury. The stromal vascular fraction (SVF) is a newer material in stem cell therapy that is made up of stem cells harvested from adipose tissue, which has been shown to promote the healing of refractory wounds of different causes. In this study, SVF was isolated from patients with radiation-induced skin injury. Adipose-derived stem cells (ADSCs) accounted for approximately 10% of the SVF by flow cytometry. Compared with the control group of rats, administration with SVF attenuated the skin injury induced by electron beam radiation. The effect of SVF on the human skin fibroblast microenvironment was determined by proteomic profiling of secreted proteins in SVF-co-cultured human skin fibroblast WS1 cells. Results revealed 293 upregulated and 1,481 downregulated proteins in the supernatant of SVF-co-cultured WS1 cells. WS1 co-culture with SVF induced secretion of multiple proteins including collagen and MMP-1. In the clinic, five patients with radiation-induced skin injury were recruited to receive SVF transfer-based therapy, either alone or combined with flap transplantation. Autogenous SVF was isolated and introduced into a multi-needle precision electronic injection device, which automatically and aseptically distributed the SVF to the exact layer of the wound in an accurate amount. After SVF transfer, wound healing clearly improved and pain was significantly relieved. The patients' skin showed satisfactory texture and shape with no further wound recurrence. Our findings suggest that transplantation of SVF could be an effective countermeasure against severe radiation-induced skin injury.

严重的辐射性皮肤损伤是肿瘤放疗和核事故的并发症。细胞疗法是一种潜在的治疗辐射引起的皮肤损伤的方法。基质血管成分 (SVF) 是干细胞治疗中的一种新材料，由从脂肪组织中提取的干细胞组成，已被证明可以促进不同原因的难治性伤口的愈合。在这项研究中，SVF 是从辐射引起的皮肤损伤患者中分离出来的。通过流式细胞术，脂肪干细胞 (ADSCs) 约占 SVF 的 10%。与对照组大鼠相比，SVF给药减轻了电子束辐射引起的皮肤损伤。SVF 对人皮肤成纤维细胞微环境的影响是通过对 SVF 共培养的人皮肤成纤维细胞 WS1 细胞中分泌蛋白的蛋白质组学分析来确定的。结果显示 SVF 共培养 WS1 细胞的上清液中有 293 个上调蛋白和 1,481 个下调蛋白。WS1 与 SVF 共培养诱导多种蛋白质的分泌，包括胶原蛋白和 MMP-1。在临床上，招募了 5 名辐射性皮肤损伤患者接受基于 SVF 转移的治疗，单独或联合皮瓣移植。自体 SVF 被分离并导入多针精密电子注射装置，该装置自动无菌地将 SVF 以准确的量分配到伤口的确切层。SVF转移后，伤口愈合明显改善，疼痛明显减轻。患者皮肤的质地和形状令人满意，没有进一步的伤口复发。我们的研究结果表明，移植 SVF 可能是对抗严重辐射引起的皮肤损伤的有效对策。