The fluorescent probes represent an important tool in the biological study, in fact characterization of cellular structures and organelles are an important tool-target for understanding the mechanisms regulating most biological processes. Recently, a series of polyamino-macrocycles based on 1,4,7,10-tetraazacyclododecane was synthesized, bearing one or two NBD units (AJ2NBD·4HCl) useful as sensors for metal cations and halides able to target and to detect apolar environment, as lipid membranes. In this paper, we firstly illustrate the chemical synthesis of the AJ2NBD probe, its electronic absorption spectra and its behavior regarding pH of the environment. Lack of any cellular toxicity and an efficient labelling on fresh, living cells was demonstrated, allowing the use of AJ2NBD in biological studies. In particular, this green fluorescent probe may represent a potential dye for the compartments involved in the endosomal/autophagic pathway. This research’s field should benefit from the use of AJ2NBD as a vesicular tracer, however, to ensure the precise nature of vesicles/vacuoles traced by this new probe, other more specific tests are needed.

中文翻译：

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基于多氮杂大环衍生物的新型囊泡运输荧光探针的合成和生物学表征


荧光探针是生物学研究中的重要工具，事实上，细胞结构和细胞器的表征是了解调节大多数生物过程的机制的重要工具目标。最近，合成了一系列基于1,4,7,10-四氮杂环十二烷的聚氨基大环化合物，带有一个或两个NBD单元（AJ2NBD·4HCl），可用作金属阳离子和卤化物的传感器，能够靶向和检测非极性环境，如脂质膜。在本文中，我们首先阐述了 AJ2NBD 探针的化学合成、其电子吸收光谱及其对环境 pH 的影响。经证明，AJ2NBD 不存在任何细胞毒性，并且可以对新鲜活细胞进行有效标记，从而允许在生物学研究中使用 AJ2NBD。特别是，这种绿色荧光探针可能代表内体/自噬途径所涉及的区室的潜在染料。该研究领域应该受益于使用 AJ2NBD 作为囊泡示踪剂，但是，为了确保这种新探针追踪的囊泡/液泡的精确性质，还需要其他更具体的测试。