In a dual approach, laser ablation-inductively coupled plasma-mass spectrometry was applied to investigate spleen samples of rats after intratracheal instillation of polyvinylpyrrolidone-coated gold nanoparticles. First, spatially resolved imaging analysis was deployed to investigate gold translocation from the lungs to the spleen and to investigate the distribution pattern of gold in the spleen parenchyma itself. Using the same instrumental setup, laser ablation-inductively coupled plasma-mass spectrometry in single particle mode was applied to determine the species of translocated gold. Single particle analysis allows the determination of particle size distributions and therefore to distinguish between ionic species, intact nanoparticles, and agglomerates. A translocation of instilled gold from the lungs to the spleen was demonstrated for gold nanoparticles of 30 and 50 nm diameter. Furthermore single particle analysis revealed the translocation of intact gold nanoparticles in a non-agglomerated state.

中文翻译：

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通过激光消融-电感耦合等离子体质谱法对气管内滴注后大鼠脾脏中金纳米颗粒的空间和尺寸分辨分析。

在双重方法中，应用激光消融电感耦合等离子体质谱法研究气管内滴注聚乙烯吡咯烷酮涂层金纳米粒子后大鼠的脾脏样本。首先，采用空间分辨成像分析来研究金从肺到脾的易位，并研究金在脾实质中的分布模式。使用相同的仪器设置，在单粒子模式下应用激光烧蚀电感耦合等离子体质谱法来确定易位金的种类。单粒子分析允许确定粒子大小分布，因此可以区分离子种类、完整的纳米粒子和附聚物。直径为 30 和 50 nm 的金纳米粒子证明了注入的金从肺转移到脾脏。此外，单粒子分析揭示了完整的金纳米粒子在非聚集状态下的易位。