Qilong capsule (QLC) is a well-known Traditional Chinese Medicine, and it has a long history for the treatment of ischemic stroke. Its major ingredients are saponins, such as paeoniflorin, amygdalin and calycosin-7-glucoside, contributing to vasodilation function. In this study, a simple, rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry method was developed to determine three bioactive ingredients in rat plasma after oral administration of QLC. A simple acetonitrile precipitation method was introduced during the sample preparation. Chromatographic separation was performed on a Shiseido CAPCELL PAC MGIII-C18 column using a gradient elution with acetonitrile and water (0.1% formic acid) as a mobile phase, and the chromatographic separation was 5 min. The methodological evaluation showed that this method had a high sensitivity (the lower limit of quantification was 1 ng/mL for calycosin-7-glucoside, 5 ng/mL for paeoniflorin, 5 ng/mL for amygdalin), satisfactory accuracy (relative error ≤ ±15%) and precision (relative standard deviation ≤15%). Then, the analytical method was applied to the pharmacokinetic study in rats following oral administration of the extracts in QLC. Meanwhile, the pharmacokinetic parameters of complex system in QLC were analyzed and the potential interaction between ingredients was explored. The present quantification method and pharmacokinetic study will provide a meaningful reference for the formulas research of QLC in the treatment of ischemic stroke. Highlight

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LC-MS/MS研究芪龙胶囊中三种成分的药代动力学特征及其在大鼠体内的潜在相互作用

芪龙胶囊（QLC）是著名的中药，用于治疗缺血性脑卒中有着悠久的历史。其主要成分为皂甙，如芍药苷、苦杏仁苷和毛蕊花苷-7-葡萄糖苷，有助于血管舒张功能。本研究开发了一种简单、快速、灵敏的高效液相色谱-串联质谱法，用于测定口服QLC后大鼠血浆中的三种生物活性成分。在样品制备过程中引入了一种简单的乙腈沉淀法。色谱分离采用资生堂CAPCELL PAC MGIII-C18色谱柱，以乙腈和水（0.1%甲酸）为流动相梯度洗脱，色谱分离时间为5 min。方法学评价表明，该方法灵敏度高（定量下限为花萼甙7-葡萄糖苷1 ng/mL，芍药甙5 ng/mL，苦杏仁苷5 ng/mL），准确度满意（相对误差≤ ±15%）和精密度（相对标准偏差≤15%）。然后，将该分析方法应用于大鼠口服给药QLC提取物后的药代动力学研究。同时，分析了QLC中复杂系统的药代动力学参数，探讨了成分之间潜在的相互作用。本定量方法和药代动力学研究将为QLC治疗缺血性脑卒中的方剂研究提供有意义的参考。强调 苦杏仁苷为 5 ng/mL），准确度（相对误差 ≤ ±15%）和精密度（相对标准偏差 ≤15%）令人满意。然后，将该分析方法应用于大鼠口服给药QLC提取物后的药代动力学研究。同时，分析了QLC中复杂系统的药代动力学参数，探讨了成分之间潜在的相互作用。本定量方法和药代动力学研究将为QLC治疗缺血性脑卒中的方剂研究提供有意义的参考。强调 苦杏仁苷为 5 ng/mL），准确度（相对误差 ≤ ±15%）和精密度（相对标准偏差 ≤15%）令人满意。然后，将该分析方法应用于大鼠口服给药QLC提取物后的药代动力学研究。同时，分析了QLC中复杂系统的药代动力学参数，探讨了成分之间潜在的相互作用。本定量方法和药代动力学研究将为QLC治疗缺血性脑卒中的方剂研究提供有意义的参考。强调 分析了QLC中复杂系统的药代动力学参数，探讨了成分之间潜在的相互作用。本定量方法和药代动力学研究将为QLC治疗缺血性脑卒中的方剂研究提供有意义的参考。强调 分析了QLC中复杂系统的药代动力学参数，探讨了成分之间潜在的相互作用。本定量方法和药代动力学研究将为QLC治疗缺血性脑卒中的方剂研究提供有意义的参考。强调